胆系恶性肿瘤VEGF,bFGF的表达与微血管计数的关系

The relationships between the expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and the counts of microvessel (MV) in biliary tract carcinoma

目的 研究胆囊癌和胆管癌组织中微血管 (microvessel,MV)计数和血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)、碱性成纤维细胞生长因子 (basicfibroblastgrowthfactor,bFGF)表达及其意义。方法 以ABC免疫组化法对 40例胆囊癌和 42例胆管癌组织常规石蜡包埋切片分别检测MV、VEGF和bFGF。结果 40例胆囊癌MV定量为 (6 6± 2 7)个 /HP ,VEGF、bFGF阳性率分别5 0 % (2 0 / 40 )和 5 3% (2 1/ 40 ) ;42例胆管癌MV定量为 (75± 19)个 /HP ,VEGF、bFGF阳性率分别为 5 5 %(2 3/ 42 )和 5 7% (2 4/ 42 )。高分化和未转移胆囊癌MV定量及VEGF、bFGF阳性率明显低于低分化腺癌〔(5 0± 2 4)vs.(89± 2 6 ) ,P <0 0 1;39%vs.6 7% ,P >0 0 5 ;39%vs.76 % ,P <0 0 5〕和转移癌〔(4 8± 9)vs.(86± 30 ) ,P <0 0 1;32 %vs .72 % ,P <0 0 5 ;36 %vs.72 % ,P <0 0 5〕 ;高分化和未转移胆管癌MV定量及VEGF、bFGF阳性率明显低于未分化癌〔(6 6± 15 )vs .(86± 2 3) ,P <0 0 5 ;41%vs.86 % ,P <0 0 5 ;35 %vs .76 % ,P <0 0 5〕和转移癌〔(6 3± 17)vs.(90± 15 ) ,P <0 0 1;40 %vs.76 % ,P <0 0 5 ,44 %vs .76 % ,P <0 0 5〕。VEGF(+ )、或bFGF(+ )或VEGF(+ )bFGF(+ )

Objectives[WT5”BZ] To study on the counts of MV and the expressions of VEGF, bFGF and their relationships in gallbladder carcinoma and extrahepatic cholangiocarcinoma.[WT5”HZ] Method[WT5”BZ] The stains of MV, VEGF and bFGF were completed by the immunohistochemical method of avidin-bitin complex in routine paraffin-embeded sections.[WT5”HZ] Results[WT5”BZ] The quantity of MV was 66±27 /HP,and the positive rate of VEGF、bFGF was 50% and 53%, respectively in 40 cases with gallbladder carcinoma. The quantity of MV was 75±19/HP,and the postive rate of VEGF、bFGF was 55% and 57%,respectively in 42 cases with extrahepatic cholangiocarcinoma. The quantities of MV and the positive rates of VEGF、bFGF were significantly lower in cases of well-differentiation and without metastasis than that of poorly-or undifferentiation and with metastasis. The quantities of MV were significantly higher in the cases of VEGF(+), bFGF (+) or VEGF(+)bFGF(+)than that of VEGF(-)、or bFGF(-),or VEGF(-)bFGF(-).The positive correlations were found among the scores of the positive cell rates of VEGF, bFGF and the counts of MV in gallbladder carcinoma and extrahepatic cholangiocarcinoma.[WT5”HZ] Conclusions[WT5”BZ] The counts of MV and the expression of VEGF, bFGF closely related to the biological behaviors, metastasis and prognosis of gallbladder carcinoma and extrahepatic cholangiocarcinoma. VEGF and bFGF play important role in the angiogenesis of biliary tract carcinoma. [WT5”HZ]