电针内关对家兔心肌缺血再灌注损伤延迟保护作用的研究

Delayed Protective Effects of Electroacupuncturing Neiguan Points on Myocardial Ischemia/ Reperfusion Injury in Rabbits

目的:探讨针刺内关对心肌缺血再灌注(I/R)损伤是否具有延迟保护作用。方法:50只家兔随机分为对照组、I/R模型组、缺血预适应(IP)组、内关组和足三里组。针剌内关和IP 24h后,造成I/R损伤模型,比较各组对心肌I/R损伤的影响。结果:针刺内关和缺血预适应24h后均可促进心肌缺血再灌注损伤家兔心电图STⅡ的恢复;降低CK、LDH和MDA;升高心肌组织内ATP、ADP、AMP含量。结论:二者对心肌缺血再灌注损伤均具有延迟保护作用,其保护效果缺血预适应优于针刺内关。

Objective:To further investigate the delayed protective effect of acupuncture treatment on myocardial ischemia and reperfusion injury. Methods:50 rabbits were divided into 5 groups:the control group, the model group, Ischemic Preconditioning (IP) group, Neiguan point group and Zusanli point group. After acupuncturing Neiguan point and IP 24 hours later, making a model of I/R, compare their influence on myocardial ischemia and reperfusion injury. Results:After electroacupuncturing Neiguan point and IP 24 hours later, both of them could significantly decrease the elevated degree of STⅡ induced by I/R and promote the recovery of STⅡ-segment;Both of them could decrease the level of CK, LDH and MDA;24 hours later IP could raise the activity of SOD significantly, but electroacupuncturing Neiguan point inhibit it;Both of them could increase the level of Adenine Nucleotides. Conclusion:Both electroacupuncturing Neiguan point and IP show the delayed protective effect on myocardial I/R injury, but the effect of IP is superior to electroacupuncturing Neiguan.