异丙酚对系统性炎性反应综合征大鼠免疫因子的影响

Effect of propofol on changes in cytokines in rats with systemic inflammatory response syndrome

目的 探讨免疫因子TNF-α、IL-6、IL-8与系统性炎性反应综合征(SIRS)发生发展的相关性,探讨不同剂量异丙酚对内毒素(LPS)诱导的SIRS大鼠血清中免疫因子的影响。方法Wistar大鼠56只,随机分成四组(n=14)。A:对照组;B:模型组,腹腔注射LPS(1mg·kg-1);C:B+异丙酚(75μg·kg-1·min-1×4h静脉注射;D:B+异丙酚(150μg·kg-1·min-1×4h静脉注射。分别取实验前、实验1h、2h、3h、4h时相股静脉血,用细胞毒染料染色法或ELISA法测血清中TNF-α、IL-6、IL-8浓度。结果 在内毒素诱导下,循环中的TNF-α、IL-6 、IL-8浓度均显著升高。异丙酚能有效降低上述免疫因子的血清浓度,并可延迟血清TNF-α、IL-8升高的出现时相。结论 异丙酚早期应用可控制免疫因子反应,对SIRS的发生有一定预防作用。

Objective To investigate the effect of different doses of propofol on cytokines-tumor necosis factor-α (TNF-α), interleukin-6 ( IL-6 ), interlenkin-8 ( IL-8 ), during systemic inflammatory response syndrome (SIRS) .Methods Fifty-six male Wistar rats weighing 190-220g were anesthetized with intraperitoneal 2.5% pentobarbital 30mg.kg-1 . The animals were randomly divided into 4 groups of 14 animals each: group A received intraperitoneal saline 10ml.kg-1 followed by intravenous infusion of saline (2ml.h-1 ); group B received intraperitoneal LPS 1mg.kg followed by intravenous infusion of saline(2ml.h-1 ) ; in group C propofol was infused iv at 75μg.kg-1.min for 4h after intraperitoneal LPS injection; in group D propofol was infusion iv at 150μg.kg-1 .min-1 for 4h after intraperitoneal LPS injection. Blood samples were taken before experiment and 1, 2, 3, 4h after saline or propofol infusion for determination of serum TNF-α (cytotoxin stain method) and IL-6, IL-8 (ELISA) concentration.Results Serum concentrations of TNF-α, IL-6 and IL-8 induced by LPS increased significantly in group B as compared with those in group A. Propofol decreased the level of cytokines and delayed the peak values of TNF-α and IL-8. Conclusion Propofol used in the early stage of SIRS has some protective efficacy.