BMP_2在大鼠牙髓损伤修复中的表达及其对牙髓细胞的生物学效应

Expression patterns of bone morphogenetic protein-2 during the healing of injured dental pulp in vivo and its biological effects on proliferation and differentiation of human dental pulp cells in vitro

目的 从体内体外两方面探讨BMP2 在牙髓损伤修复 ,尤其是牙髓细胞分化过程中的作用。方法 建立大鼠牙髓损伤修复模型和体外人牙髓细胞连续培养 ,采用免疫组织化学、MTT法和酶动力学等方法 ,检测BMP2 在体内牙髓组织损伤修复过程中的表达 ,并比较不同浓度rhBMP2 对体外人牙髓细胞连续培养各阶段细胞增殖活性及ALP活性的影响。结果 体内牙髓组织损伤修复过程中存在BMP2 的表达 ,牙髓组织损伤修复的不同阶段其表达部位和强度发生变化 ;rhBMP2 可提高体外培养人牙髓细胞的增殖活性 ,与其本身浓度有关 ,并可促进人牙髓细胞ALP活性 ,呈浓度依赖性 ;结论 BMP2 是参与牙髓损伤修复 ,尤其是牙髓细胞的增殖和分化的重要因子之一。

Objective To explore the roles of bone morphogenetic protein 2 (BMP 2) in the healing of injured dental pulp in vivo and in vitro . Methods The expression patterns of BMP 2 during the healing of injured dental pulp were studied with immunohistochemistry and the effects of recombinant human bone morphogenon 2 (rhBMP 2) on proliferation and differentiation of cultured human dental pulp cells were also studied in this experiment. Results The results clearly demonstrated that BMP 2 were expressed in the course of rat dental pulp healing, its expression sites and levels of their expression were changed with different phases; rhBMP 2 increased the proliferation of dental pulp cells and ALP activity in a dose dependent manner. Conclusion BMP 2 plays an important role in the healing of injured dental pulp, which may be due to promote the differentiation of dental pulp cells to odontoblasts.