1型糖尿病儿童氧化应激水平的研究

Status of oxidative stress in children with type 1 diabetes mellitus

目的比较1型糖尿病患儿与健康儿童血清中氧化应激标志物水平,探讨1型糖尿病患儿氧化及抗氧化能力的改变。方法选取62例1型糖尿病患儿为病例组,36例健康儿童为对照组。病例组根据病程分为初发组和病程稍长组;根据感染情况分为感染组和非感染组。测定各组血清中氧化标志物丙二醛(MDA),一氧化氮(NO)及抗氧化标志物超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GPx)水平,同时常规测定病例组空腹血糖、C-肽、糖化血红蛋白(HbA1C)、尿素氮,并对结果进行差异性和相关性分析。结果1型糖尿病患儿血清MDA,NO,SOD水平显著高于对照组(P<0.05),GPx水平显著低于对照组(P<0.01)。初发组MDA、NO、SOD水平显著高于对照组(P<0.05),GPx水平显著低于对照组(P<0.01)。病程稍长组SOD水平与对照组比较、感染组与非感染组间各指标比较,差异均无统计学意义(P>0.05)。MDA水平与HbA1C水平呈显著正相关(r=0.377,P<0.01)。结论 1型糖尿病患儿发病初期及病程进展过程中存在明显的氧化应激水平升高和抗氧化保护能力下降,提示氧化应激可能在儿童1型糖尿病中起着重要作用。

Objective To compare the serum levels of oxidative stress index in type 1 diabetic children and healthy children,and explore the changes of oxidative and anti-oxidative ability of children with type 1 diabetes mellitus. Methods A total of 62 type 1 diabetic children were selected as study cases,and 36 healthy children as normal controls.According to duration and infected situation,the study cases was divided into onset group and little long-term group,infected group and un-infected group.Serum levels of oxidative parameters:malondialdehyde(MDA),nitric oxide(NO),and anti-oxidative parameters:glutathione peroxidase(GPx),superoxide dismutase(SOD)were measured.At the same time,fasting blood glucose,c-peptide,glycosylated hemoglobin(HbA1c),blood urea nitrogen in study cases were detected.The significance and correlation among these parameters were analyzed. Results The serum MDA,NO,SOD levels in study cases were significantly higher than controls(P<0.05),while the levels of serum GPx level in cases was significantly lower than controls(P<0.05).In the onset group,MDA,NO,SOD were significantly higher than controls(P<0.05),GPx was significantly lower than controls,too(P<0.05).There was no significance between infected and un-infected groups(P>0.05).There was a positive correlation between levels of MDA and levels of HbA1c(r=0.376,P<0.01). Conclusions The significant increase of oxidative stress and decrease of anti-oxidative capacity occurr at onset and in the development of type 1 diabetes mellitus in children.Oxidative stress may take the important role in type 1 diabetes mellitus of children.