小儿肺炎支原体感染耐药情况回顾分析

Retrospective analysis of drug resistance of mycoplasma pneumoniae infection in children

目的了解2015-2016年北京地区儿童肺炎支原体(MP)耐药情况及耐药机制分析。方法采集2015-2016年首都医科大学附属北京朝阳医院儿科收治的,具有发热、咳嗽或咽痛等呼吸道感染症状之一的,门诊和住院的89例患儿的呼吸道咽拭子标本,进行MP培养与药敏试验,采用巢式PCR扩增法鉴定MP耐药位点。结果 53例MP阳性,感染率为59%,18株为敏感株,耐药率66%,32株存在23SrRNA结构域Ⅴ区A2063G点突变。3株2063、2064位点均有突变。结论北京地区肺炎支原体感染率高,对大环内酯类抗生素耐药率上升趋势,耐药机制主要为23SrRNA结构域Ⅴ区点突变。

Objective To investigate the prevalence and mecharism of drug resistance of mycoplasma pneumoniae(MP)of children in Beijing area in 2015-2016.Method A total of 89 respiratory throat swab specimens were collected from children with fever,cough or sore throat and other respiratory tract infection symptoms,admitted in Beijing Chaoyang Hospital Affiliated to Capital Medical University in 2015-2016.These cases were assayed mycoplasma culture and drug sensitivity test and were amplified by nested PCR method for identification of MP resistance loci.Results 53 cases were positive of mycoplasma pneumonia,with infection rate of 59%.18 cases were sensitive strains and drug resistance rate was 66%.32 strains showed A2063 Gpoint mutation in 23 SrRNA domain V district.Both A2063G and A2064G point mutation existed in 3cases.Conclusions Mycoplasma pneumoniae infection rate in Beijing area is prevalent.The macrolide resistance rate is rising and the main resistance mechanism is 23 SrRNA domain V point mutation.