肠道病毒71型感染乳鼠脑组织白介素1β与白介素6水平的研究

Study on the levels of interleukin 1 beta and interleukin-6 in brain tissue of mice infected with enterovirus type 71

目的检测肠道病毒71型(EV71)感染乳鼠脑组织白介素1β(IL-1β)与白介素6(IL-6)水平变化,探讨EV71致中枢损害可能的发病机制。方法 1日龄120只ICR乳鼠随机分为感染组及对照组。腹腔注射法建立EV71感染乳鼠模型。荧光定量RT-PCR法检测脑组织中EV71载量。光镜观察脑组织病理变化。酶联免疫吸附法(ELISA)检测脑组织匀浆液中IL-6与IL-1β含量。结果感染组3d、5d与7d乳鼠脑组织匀浆IL-1β浓度较对照组明显升高(P<0.01),感染组3d、5d、7d与10d乳鼠脑组织匀浆IL-6浓度较对照组明显升高,差异有统计学意义(P<0.01),感染组1d与14d乳鼠脑组织匀浆IL-1β及IL-6浓度与对照组相比差异无统计学意义(P>0.05)。结论 EV71感染乳鼠早期脑组织中IL-1β及IL-6含量明显升高,其介导的免疫反应可能是EV71致中枢损害可能的机制之一。

Objective To detect the changes of interleukin 1 beta(IL-1β)and interleukin 6(IL-6)level in the brain tissue of mice infected with enterovirus type(EV71),and to explore the possible pathogenesis of central lesion caused by EV71 infection.Methods Totally 120 one-day-old ICR mice were divided into infection group and control group.EV71 infected mice model was established by intraperitoneal injection.Fluorescence quantitative RT-PCR technique was used to detect the viral load of EV71 in the brain.Pathological changes on brain tissues were observed through hematoxylin-eosin staining.Enzyme linked immunosorbent assay(ELISA)was used to detect the content of IL-6 and IL-1βin brain homogenate.Results The level of IL-1β was significantly higher in infection group at 3,5 and 7 days compared with that in control group(P<0.01).The level of IL-6 was significantly higher in infection group at 3,5,7 and 10 day compared with that in control group(P<0.01).However,no significant difference was observed in the level of IL-1β and IL-6 in infection group at 1,7,10 and14 days compared with those in control group(P>0.05).Conclusion The content of IL-1 and IL-6 in brain tissue of mice infected with EV71 was significantly increased,and the immune response mediated by IL-1β and IL-6 may be one of the possible mechanisms of CNS damage.