武汉市呼吸道感染儿童博卡病毒的检测及临床分析

Detection and clinical characteristics analysis of human bocavirus in children for acute respiratory infection in Wuhan

目的分析武汉市呼吸道感染儿童博卡病毒(HBoV)感染的临床特点,为临床治疗提供理论依据。方法收集2014年12月1日-2015年12月1日期间在本院儿科住院的365例急性呼吸道感染患儿为研究对象,在疾病急性期用一次性吸痰管采集患儿鼻咽分泌物(NPA)标本液2ml。先进行7种常见呼吸道病毒的筛查,然后采用PCR方法检测HBoV基因,对阳性标本进行序列验证及分析。结果1)对365份标本进行免疫荧光检测,结果显示呼吸道合胞病毒(RSV)的阳性检出率最高18.90%(69/365),其他依次为腺病毒(ADV)阳性15例(4.11%),副流感病毒Ⅲ(PIV-Ⅲ)阳性8例(2.20%),甲型流感病毒(IFV-A)阳性5例(1.37%),乙型流感病毒(IFV-B)阳性3例(0.82%),副流感病毒Ⅱ型(PIV-Ⅱ)阳性2例(0.55%)。2)365例标本中,32例(8.77%)出现HBoV的PCR扩增产物,均为单一条带,大小约为354bp,与预期的扩增片段大小相符合,显示为HBoV阳性。32例阳性标本进行测序后经Blast分析,HBoV1型28例(7.67%),HBoV2型3例(0.82%),HBoV3型1例(0.27%)。3)32例HBoV阳性患儿中,其中男19例,女13例,男女比例为1.47∶1;年龄3~13个月,平均6.87个月。结论 HBoV感染是引起武汉市小儿呼吸道感染的重要病毒之一,主要以HBoV1型感染为主,而且多为混合感染。应加强对婴幼儿的预防保健工作,以便进行合理用药、早期治疗。

Objective To study the clinical and molecular epidemiologic characteristics of human bocavirus in children for acute respiratory infoction in Wuhan.Methods Nasopharyngeal aspiration samples and throat swabs were collected from 365 children admitted from December 2014 to December 2016.PCR WSS was used to screen HBoV,which amplified a518 bp fragment of the partial gene.Furthermore,a standard reverse transcription-PCR was used to screen for other common respiratory viruses.Results The positive rate of respiratory syncytial virus was the highest(69 cases,18.90%),followed by adenovirus positive in 15 cases(4.11%),parainfluenza virusⅢ positive in 8 cases(2.20%),influenza A virus positive in5 cases(1.37%),influenza B virus positive in 3 cases(0.82%),parainfluenza virus typeⅡ positive in 2 cases(0.55%).2)In 365 samples,32 cases showed HBoV PCR amplification products,with single band and about 354 bp in size,which was consistent with the expected size of the amplified fragments.The positive rate of HBoV was 8.77%(32/365).HBoV1 type(28 cases,7.67%),HBoV2 type(0.82%)and HBoV3 type(0.27%)were identified by Blast analysis after 32 positive samples were sequenced.3)32 cases of HBoV-positive children included 19 males and 13 females,with male to female ratio of 1.47∶1;The children were from 3 to 13 months old,with average age of 6.87 months.Conclusions HBoV infection,mainly HBoV1-based infection and mixed infection,is one of the major respiratory tract infection virus in children of Wuhan city.It is supposed to strengthen the prevention and care of infants and young children in order to carry out rational drug use and early treatment.