新生儿HIE外周血T细胞亚群、IL-6、IL-8水平变化及临床意义探讨

THE CLINICAL SIGNIFICANCE OF T LYMPHOCYTIC SUBGROUP AND BL-6, IL-8 LEVELS IN PERIPHERAL BLOOD OF NEONATAL HIE

目的:观察新生儿HIE外周血T细胞亚群、IL-6、IL-8水平变化,并探讨其临床意义。方法:收集50例HIE患儿和30例正常新生儿外周血,采用双色免疫荧光标记法及流式细胞仪检测CD3+、CD4+、CD8+T细胞免疫表型,采用酶标免疫吸附法检测IL-6、IL-8。记录HIE患儿临床资料。结果:HIE组外周血CD3+、CD4+、CD8+细胞水平低于对照组,IL-6、IL-8高于对照组,差异有高度显著性（P<0.01）;不同病情、不同转归HIE组CD3+、CN4+、CD8+、IL-6差异有高度显著性（P<0.01）,即CD3+、CD4+、CD8+降低、IL-6升高越显著者,病情越重,预后越差。结论:新生儿HIE免疫功能受损,CD3+、CD4+、CD8+细胞和IL-6、IL-8水平对临床早期判断病情严重性、估计预后、指导治疗有一定意义。

To study the change of T cell subgroup and IL-6, IL-8 levels in peripheral blood of neonates with hy-poxic ischemic encephalopathy(HIE) ,and their relationship bewith with HIE .Method Peripheral blood of 50 neonates HIE and 30 normal neonates were collected. Immunophenotypes of T cell were determinedby double-innumofluorescence labeled assay with flow cytometry. The levels of IL-6 and IL-8 were determined by enzyme-linked immunosorbent assay(ELISA) .Clinical data of HIE neonates were collected and analysed. Results showed peripheral blood CD3+ , CD4+ , CD8+ T cell levels in HIE were significantly lower than that in normal control. IL-6, IL-8 levels significantly higher in HIE neonates than those in normal control. The levels of CD3+ ,CD4+ ,CD8+ T cell and IL-6 were significantly different in different disease severity and different prognosis cases.That was,The low CD3+ ,CD4+ , CD8+ levels and high IL-6 level wereassociated with more serious HIE and poor prognosis. We Concluded that immunological function in neonatal HIE was impaired.The levels of CD3+ ,CD4+ ,CD8+ T cell and IL-6, IL-8 could be a marker for the disease severity assessing and outcome predicting.