电针对狗血浆、胃粘膜组织中内皮素含量的影响及意义

Effects of Electroacupuncture on Plasma and Gastric Mucosal Levels of ET in Dogs

目的 :观察电针对狗胃粘膜血流量的调控作用及其与血浆、胃粘膜组织中内皮素水平变化的关系 ,以探讨电针对胃粘膜保护作用的机制。方法 :将 2 0条狗随机分为四组 :空白对照组、非经非穴组、“上巨虚”组、“足三里”组 (每组 5条 )。采用激光多普勒血流仪监测狗胃粘膜血流量的变化 ,同步测定血浆及胃粘膜组织中内皮素含量 ,并观察其变化规律。结果 :给予电针 3 0min ,停针 3 0min后“足三里”组胃粘膜血流量较针前显著升高 (P <0 .0 1 ) ,血浆及胃粘膜组织中内皮素含量显著下降 (P <0 .0 1 ) ,电针后“上巨虚”组仅血浆内皮素含量较针前下降 (P <0 .0 5) ,而胃粘膜血流量及胃粘膜组织内皮素含量无显著变化 ,其他组各监测指标无显著变化。结论 :电针可使狗胃粘膜血流量增加 ,对胃粘膜具有保护作用 ,这种变化与影响胃粘膜血流量某些脑肠肽的含量改变有关 。

Objective: To investigate the regulation action of electroacupuncture(EA)on gastric mucosal blood flow (GMBF) and its relation to the contents of plasma and gastric mucosal ET in dogs. Methods: Twenty mongrel dogs (body weight 15～20 kg) anesthetized with pentobarbital (1 mg/kg) were randomly and equally divided into control group,non-acupoint group,'Shangjuxu' group and 'Zusanli' group. 'Shangjuxu'(ST 37), 'Zusanli'(ST 36) and non-acupoint (the midpoint between the small head of the fibula and the external malleolus, 1 cm to the lateral side of the fibula) were punctured and stimulated with an EA therapeutic apparatus (50 Hz, intermittent waves, 20 V) for 30 min. GMBF (gastric body) was measured by using a laser doppler flowmeter(LDF),and plasma ET contents were detected with radioimmunoassay method. Results: In 'Zusanli' group,30 min after EA stimulation and cease of EA, GMBF increased significantly (P<0.05, 0.01); while no significant changes were found after EA in 'Shangjuxu', non-acupoint and control groups. Simultaneously, 30 min after cease of EA, marked decrease was observed in plasma ET content (P<0.01) and gastric mucosa ET content (P<0.01) in both 'Zusnanli' and 'Shangjuxu' groups, whereas no significant changes were observed in control and non-acupoint groups in plasma and gastric mucosal tissue. Conclusion: EA at 'Zusanli' (ST 36) can evidently make GMBF increase, suggesting a protective effect of EA on gastric mucous. This effect is probably related to EA-induced changes of plasma and gastric mucosa brain-gut peptide and the effect of EA of different meridian acupoints is of a certain specificity.