针刺防治实验性动脉再狭窄及其机制的初步研究

Preliminary Study on Mechanisms of Acupuncture in Preventing and Treating Arterial Restricture in the Rat

目的 :探讨针刺治疗动脉再狭窄的作用机理。方法 :用空气干燥法诱发大鼠颈总动脉再狭窄模型 ,以复方丹参注射液作对照 ,观察了针刺对动脉再狭窄模型大鼠右颈总动脉去内皮损伤的内膜增生及纤溶活性的影响。结果 :针刺能抑制动脉去内皮损伤的内膜增生 ,升高模型大鼠血清中组织型纤溶酶原激活剂 (t PA)的活性 ,降低纤溶酶原激活剂抑制物 (PAI)的活性 ,从而影响动脉再狭窄的病理进程。结论

Objective: To explore the mechanism of acupuncture in preventing and treating arterial restricture. Methods: Twenty-four SD rats were used in the present study. Rat carotid artery restricture model was established in accordance with Fishman's method. These rats were randomly and evenly divided into control, acupuncture and Danshen (Radix Salviae Miltiorrhizae, 丹参)groups. 'Neiguan'(PC 6), 'Jueyinshu'(BL 14), 'Xinshu'(BL 15), 'Danzhong'(CV 17) and 'Zusanli'(ST 36) were punctured using filiform needles and stimulated with uniform reinforcing-reducing method. The treatment was conducted once daily beginning from the first 2 days on before operation (establishing model) to the 14th day after operation. The 8 rats of Danshen group were fed with Danshen injectio (1.44g/kg/d) once daily and those of control group fed with normal saline (10 mL/kg/d) once daily. The duration of Danshen and saline treatment was the same to that of acupuncture group. Serum t-PA (histiocytic type plasminogen activator) and PAI (plasminogen activator inhibitor) activities were assayed with enzyme linked immunosorbent assay. Results: Electronic microscopic observation showed that arterial restricture injury included severe platelet adhesion, exfoliation of the vascular endotheliocytes, etc., suggesting success of the model. After treatment, the thickness values of the vascular endangium and the media on the injured side in control, Danshen and acupuncture groups (in each group, the tested arteries=16) were 79.86±7.88 μm and 46.88±4.60 μm; 17.04±1.22 μm and 40.25± 3.0 μm; 17.25±0.93 μm and 39.88±2.50 μm separately, presenting significant differences between Danshen or acupuncture and control groups (P<0.05～0.01). The serum t-PA and PAI and PAI/t-PA in control, Danshen and acupuncture groups were 0.15±0.08(10 3 U/L), 1.74±0.16 (10 3 U/L) and 15.16±2.37; 0.22±0.05(10 3 U/L), 1.19±0.08(10 3 U/L) and 7.6±1.67; 0.23±0.10(10 3 U/L), 1.16±0.12(10 3 U/L) and 6.4±1.59 respectively. There were significant differences between Danshen or acupuncture and control groups (P<0.05～0.01). Conclusion: Both Danshen and acupuncture can lighten vascular injury, raise t-PA activity and reduce PAI activity.