豚鼠小肠绒毛内对脂褐质类物质排泄的观察

Observation of The Lipofuscin-like Substances Eliminated by The Small Intestines of Guinea Pigs

目的 :为进一步确定小肠是否具有排泄功能。方法 :取豚鼠小肠做HE染色、PAS染色、尼罗蓝染色和Schmorl氏铁氰化钾染色 ,进行光镜观察。结果 :现在小肠绒毛顶端的结缔组织内及上皮内 ,可见HE染色呈黄褐的颗粒性物质 ,这些颗粒多为吞噬状态 ,脂褐质特异性染色呈阳性反应。结论

Objective:Further to determine that the small intestines have the function of elimination.Methods:The small intestines were stained by Hematoxylin Eosin,periodic acid Schiff reaction,Nile blue and schmorl's potassic ferricyanide reaction,and observed by light microscope.Result:There were a lot of lipofuscin like substances were found in the epithelium and connective tissues of the tips of small intestinal villi.By HE stained,the lipofuscin like substances appeared to be yellow or brown particles,most of them were presented to be phagocytized.All the particles showed the characteristics of lipofuscin positive reactions.Conclusion:The small intestines of guinea pigs may have the capability to eliminate the lipofuscin.