树舌多糖GF对HepA瘤细胞Rb基因表达的影响

Effects of Ganoderma Appanatum Polysacharides GF on Rb Gene Expression in HepA Cells

目的 探讨树舌多糖GF对小鼠HepA瘤细胞Rb基因表达的影响。方法 本实验用链霉菌抗生素蛋白 -过氧化酶免疫组化法来测定细胞中Rb蛋白含量 ,通过多媒体图像分析系统分析实验结果。结果 树舌多糖GF组、猪苓多糖组与荷瘤对照组比较 ,差异均非常显著 (P <0 .0 1) ,即树舌多糖GF、猪苓多糖能促使HepA瘤细胞中Rb基因表达明显增强 ;树舌多糖组与猪苓多糖组比较 ,差异非常显著 (P <0 .0 1) ,即树舌多糖GF更能明显增强Rb基因表达 ,优于猪苓多糖。结论 树舌多糖GF抗瘤作用机理之一是作用于抑癌基因Rb并使之表达增强。

Objective To study the effect of GAPSGF on Rb gene expression in HepA cells.Methods The level of Rb gene expression was determined by ultrasensitive TM S-P Kit.The kit was based on labeled Streptavidin-Peroxidase immunohistochemistry method.the ultimate results were gotten by multimedia colorful pathologic image procesing system.Results The average levels of Rb gene expression in GAPSGE group and PUPS group have much difference with control group.that is to say,GAPSGF and PUPS can significantly strengthen the expression of Rb gene in HepA cells.GAPSGF can more remarkably improve the levels of Rb gene expression than PUPS.Conclusion GAPSGF effect on Rb gene expression is one of the mechanisms of antitumor activity. 〔