砷化合物对健康人淋巴细胞DNA损伤差异的研究

Study on the difference in DNA damage of human lymphocyte caused by arsenicals

目的 研究 3种砷化合物对健康人淋巴细胞DNA的损伤效应间是否存在作用差异。方法 无菌分离健康人静脉血淋巴细胞 ,分别染以亚砷酸钠 (AsⅢ)、砷酸钠 (AsV)及甲基砷酸钠(MAsV)。每种处理因素均分 5个剂量组 :1、5、10、2 0、5 0 μmol/L。培养 2 4h后进行单细胞凝胶电泳实验 (SCGE) ,计数彗星细胞个数 ,测量总彗星长度。结果 除MAsV 的 1μmol/L剂量组外 ,其他各因素各剂量组彗星细胞的频数分布与对照组相比差异均有显著性 (P <0 .0 5 )。除AsV1μmol/L和MAsV1、5 μmol/L 3个剂量组外 ,其他各因素各剂量组的总彗星长度均明显高于对照组。同一处理因素不同剂量组间以及不同处理因素相同剂量组间彗星细胞的频数分布及总彗星长度差异均有显著性 (P <0 .0 5 )。砷化合物作用剂量与彗星细胞比率及总彗星长度之间均具有相关性 (rAsⅢ =0 .8134,rAsV =0 .8734,rMAsV=0 .8994 )。结论 3种砷化合物均可引起健康人淋巴细胞DNA损伤。在同一处理因素作用下 ,砷化合物作用剂量与DNA损伤效应间存在剂量 -效应关系 ;不同处理因素同一作用剂量时 ,3种砷化合物对DNA的损伤效应间存在差异 ,损伤程度表现为AsⅢ >AsV>MAsV。

Objective To explore whether there is difference in arsenicals-induced DNA damage of human lymphocyte. Methods Lymphocyte were sterilely collected from healthy donor and exposed to sodium arsenite(AsⅢ),sodium arsenate(AsV) and methyl sodium arsenate(MAsV) at 1,5,10,20 and 50 μmol/L.After incubation of 24 hours,cells were collected by centrifugation and DNA damage was detected by single cell gel electrophoresis(SCGE). Results The comet frequency distribution of all groups except 1 μmol/L group of MAsⅤ were significantly different from that of control.The comet length of all groups except 1 μmol/L group of AsV and 1,5 μmol/L groups of MAsV were significantly higher than that of control.There were correlations between the doses of arsenicals and the ratios of comet cell or length of comet(r AsⅢ=0.813 4,r AsV=0.873 4,r MAsV=0.899 4). Conclusion DNA damage in human lymphocyte were induced by all the three arsenicals.A dose-effect relationship was observed between exposure doses of the same arsenical and DNA damage.With different arsenicals but the same exposure dose,the DNA damage level was as follow:AsⅢ>AsV>MAsV.