三种汽油增氧剂对小鼠成纤维细胞DNA损伤的研究

Study on three kinds of gasoline oxygenates-induced DNA damage in mice fibroblasts

目的 比较 3种汽油增氧剂对小鼠成纤维细胞DNA的损伤作用。方法 采用单细胞凝胶电泳试验 (彗星试验 )对 3种汽油增氧剂甲基叔丁基醚 (MTBE)、无水乙醇 (EA)和碳酸二甲酯 (DMC)所致的L 92 9小鼠成纤维细胞的DNA损伤进行了研究。结果 在一定浓度范围内 (37.5 0 0～ 15 0 .0 0 0mg/ml) ,MTBE可直接引起L 92 9小鼠成纤维细胞的DNA损伤 ,出现拖尾的彗星细胞。其拖尾细胞百分率和DNA迁移长度均随受试物浓度的增加而增加 ,且有明显的剂量 -效应关系 ,即MTBE浓度从9.375mg/ml增加到 15 0 .0 0 0mg/ml时 ,彗星率从接近阴性对照组的 4 %增加到接近阳性对照组的85 % ,彗尾长度也发生了相应的改变。而EA和DMC无此关系。结论 在本试验条件下 (浓度为15 0 .0 0 0mg/ml) ,MTBE对DNA具有明显的损伤作用 ;未发现EA和DMC对DNA的损伤作用。

Objective To study DNA damage of three kinds of gasoline oxygenates. Method Single cell gel electrophoresis assay(Comet assay) was used to detect the damage effects of three gasoline oxygenates[methyl tertiary butyl ether(MTBE),ethanol anhydrous(EA) and dimethyl carbonate(DMC)] on DNA in L-929 mice fibroblasts. Results In certain concentration(37.500～150.000 mg/ml),MTBE could directly cause DNA damage of L-929 mice fibroblasts.There was obvious dose-effect relationship,ie when the concentation of MTBE was increased from 9.375 to 150.000 mg/ml,the comet rate also increased from 4% to 85%,and the length of comet tail changed correspondingly.The results of EA and DMC were negative. Conclusion Under the condition of this experiment(150.000 mg/ml),MTBE could directly cause DNA damage while the effect of EA and DMC on DNA damage was not found.