东莨菪碱和氯胺酮预防主动脉阻断所致脊髓缺血性损伤

Prevention of spinal cord ischemic injury following aortic cross-clamping using scopolamine and ketamine

目的 评价东莨菪碱和氯胺酮对主动脉阻断所致脊髓缺血性损伤保护作用。方法 2 8只新西兰大白兔随机分成 4组 :对照 (C)组、东莨菪碱 (S)组、氯胺酮 (K)组、东莨菪碱 +氯胺酮 (S +K)组。每组肾下主动脉阻断 30min ,动态监测脊髓血流量 (SCBF)、后肢运动功能、组织水含量及组织学改变。结果 再灌注 2 0h ,后肢运动功能S +K组优于C组 (P <0 0 5 ) ,脊髓水含量S +K组比C组减少 10 5 % (P <0 0 1) ,组织学改变S +K组最轻 ,C组最严重。阻断中及开放 2hS +K组SCBF比值较C组显著增加 (P <0 0 1)。

Objective This study aims at evaluating spinoprotective effects of scopolamine and ketamine on spinal cord ischemic injury following aortic cross clamping. Methods Twenty eight New Zealand rabbits were randomly divided into four equal groups: control(C), scopolamine (S), ketamine(K), scopolamine+ketamine(S+K). The infrarenal aorta of all of the animals was occluded for 30 minutes followed by observations of spinal cord blood flow(SCBF), hinder limb moto function, tissue water and histopathologic change. Results Twenty hours after reperfusion, hinder limb movement function in group S+K was better than in the control group ( P <0 05); spinal cord water content was 10.5% higher in the control group (80 4%±6 9%) than in group S+K (69 9%±0 6%, P <0 01); histopathologic change was the slightest in group S+K and the most seriousin group C. The ratio of SCBF in group S+K was significantly increased compared with the control group ( P <0 01) during clamping and 2 hours after ischmia. Conclusion Scopolamine and ketamine are more effective in preventing spinal cord ischemic injury when used together than when used separately.