咽喉部恶性黑色素瘤多药耐药基因及其基因编码产物与预后关系的研究

Correlation of the expression of MDR genes and MDR genes-coded product with prognosis in laryngopharyngeal malignant melanoma

目的 :研究咽喉部恶性黑色素瘤多药耐药 (MDR)基因胎盘型谷胱甘肽 S 转移酶 (GST π)、DNA拓朴酶Ⅱ (TopoⅡ )和MDR基因编码产物P 糖蛋白 (Pgp)的表达及其与预后的关系。 方法 :应用链霉素亲生物素 过氧化物酶标S P法检测 2 8例咽喉部恶性黑色素瘤患者Pgp、GST π和TopoⅡ的表达 ,分析MDR基因及MDR基因编码产物阳性表达率与肿瘤主要临床病理特征及预后的关系。结果 :2 8例标本中Pgp、GST π和TopoⅡ的表达率分别为 35 .7%、5 7.1%和 4 6 .4 % ,相互间差异无显著性意义 (P >0 .0 5 )。Pgp、GST π和TopoⅡ的表达与性别、年龄、肿瘤大小及Clark’s分级、Breslow’s分级无明显相关 (P >0 .0 5 ) ,与AJC分级及预后显著相关 (P <0 .0 5 )。结论 :Pgp、GST π和TopoⅡ等多因素联合作用是咽喉部恶性黑色素瘤多药耐药的主要作用机制 ,与预后相关。

Objective:To study the correlation of expression implication of MDR genes (GST π and TopoⅡ) and MDR gene coded product (Pgp) with prognosis in laryngopharyngeal malignant melanoma. Method:Using immunohistochemical S P method,expression of GST π,TopoⅡ and Pgp was detected in 28 cases of laryngopharyngeal malignant melanoma.The relationship between GST π,TopoⅡ,Pgp and clinicopathological features,prognosis of the tumors were also analyzed.Result:The positive rates of Pgp,GST π and TopoⅡ were 35.7 %, 57.1 % and 46.4 % respectively in 28 cases of laryngopharyngeal malignant melanoma,without significant difference(P> 0.05 ). The positive rates of Pgp,GST π and TopoⅡ were not correlated with sex,age,size of tumor and Clark's grade?Breslow's grade(P> 0.05 ),but AJC grade and prognosis(P< 0.05 ).Conclusion:Expression of Pgp,GST π and TopoⅡ may be the chief mechanism of multi drug resistance. Detection of multi drug resistance genes is of necessity and feasibility when predicting the effect of chemotherapy.