喉软骨支架缺损应用骨形态发生蛋白再生的实验研究

An experimental study on repair of laryngeal cartilage defect with the bone morphogenetic protein composite

目的 :观察骨形态发生蛋白 纤维蛋白复合物 (BMP FG)、骨基质明胶 (BMG)、羟基磷灰石 (HA)在行犬甲状软骨缺损移植修复中的效果 ,为寻求一种理想的移植材料提供实验依据。方法 :制备甲状软骨缺损动物模型 ,将其分为 4组 :空白组、HA组、BMG组和BMP FG组 ,分别用相应的上述材料进行修复 ,并于术后 1、2、4、8和12周随机抽取一定数量的犬取材行大体标本观察 ,光镜和电镜检测。结果 :BMP FG和BMG均能诱导缺损部位形成软骨或骨 ,但前者效果明显优于后者 ;HA不能将缺损形成良好修复。结论 :BMP FG是喉软骨缺损修复的良好材料 ,优于BMG 。

Objective:To study the effects of the bone matrix gelatin and composite of bone morphogenetic protein and fibrin glue (BMP FG) on the repair of thyroid cartilage defect. Method:Full thickness defects measuring 0.4 cm×0.4 cm were created in four sections in thyroid cartilages of 20 adult dogs. The defects were divided into 4 groups by the sections they in. Group A was empty group, without implant; Group B were repaired by implanted with hydroxylapatite; Group C with bone matrix gelatin (containing BMP); Group D with bone morphogenetic protein fibrin glue composite. The animals were killed separately at 2, 4, 12 weeks after operation. The effects of repair were analyzed by routine histological examination and electron microscope. Result:In the BMP FG implanted group,the result showed complete absorption of the implant and substitution by new formed cartilage and bone. The defects in the BMG group regenerated less cartilage and bone, the inflammation was sever. The gross appearance and histological features of HA group were similar to the empty group.Conclusion:Both BMP FG and BMG can induce cartilage and bone regeneration to repair the defect of thyroid cartilage. BMP FG has a better effect.