阿胶对染铅大鼠海马损害的拮抗作用

The antagonism of Donkey-hide gelatin against the damage of hippocampus induced by lead in rats

目的 观察和探讨阿胶对铅所致大鼠学习记忆损害的拮抗作用。方法 选用 3 6只Wistar雄性大鼠随机分为对照组、铅组、阿胶铅组 ,每组 12只。大鼠经口染铅并给予阿胶灌胃。每月用Y型迷宫试验测试学习记忆 1次。 3个月后处死大鼠 ,分别测定海马组织一氧化氮 (NO)和总抗氧化能力 (TAOC)并做病理检查。结果 (1)在Y型迷宫试验中 ,铅组大鼠学习记忆达标前所受电击次数 (错误次数 )显著高于对照组 (P <0 0 1) ;阿胶铅组大鼠所受电击次数显著低于铅组 (P <0 0 5 ;2、 3月P <0 0 1)。 (2 )铅组大鼠海马NO和TAOC显著低于对照组 (P <0 0 1) ,阿胶铅组大鼠海马NO和TAOC显著高于铅组 (P <0 0 5或P <0 0 1)。 (3 )病理检查 ,铅组大鼠海马显著萎缩 ,细胞形态不规则、排列紊乱 ,细胞变性、脱失显著 ,轴突溶解、消失 ;阿胶铅组海马病理变化不明显。结论 铅可损害海马细胞结构和功能 ,降低海马组织NO和TAOC水平 ,降低学习记忆能力 ;

Objective To observe and explore the antagonism of Donkey hide gelatin(DHG) against the impairment of learning and memory induced by lead in rats.Methods 36 male Wistar rats were chosen and divided into 3 groups at random,12 rats in every group:(1)control group:water,(2)lead group:lead [0 1g/(kg·d)],(3)lead DHG group:lead[0 1g/(kg·d)]+DHG[1 0g/(kg·d)].Wistar rats were given DHG orally after being given lead every day for three months.The ability of learning and memory of each group was measured by Y maze test.Eventually,the rats were decapitated and nitric oxide(NO) as well as total antioxidative capacity (TAOC) in hippocampus were measured immediately,in the same time,pathological examination was also made.Results (1) In Y maze test,the number of electric shocks of lead group was significantly increased in comparison with the control ( P <0 01);the number of electric shocks of lead DHG group significantly decreased in comparison with lead group (in the first month P <0 05,the second and third month P <0 01).(2)The NO and TAOC in hippocampus of lead group were significantly decreased in comparison with the control ( P <0 01),the NO and TAOC in hippocampus of lead DHG group were significantly increased in comparison with the lead group ( P <0 05 or P <0 01).(3)Pathological examination showed an appearance of atrophy,denaturation and necrosis of cells with their neurofibers dissolving and obliteration in hippocampus of lead group rats but no obviously impaired was shown in rats of lead DHG group.Conclusion The results showed that lead significantly damaged the function and structure of hippocampal cells,decreased NO and TAOC in hippocampus,and impaired the ability of learning and memory;DHG significantly decreased the impairment or damage induced by lead.