子宫营养试验检测双酚A的雌激素样活性

IMMATURE SD RAT UTEROTROPHIC ASSAY OF BISPHENOL A

目的 :采用子宫营养试验 ,检测双酚A(BisphenolA ,BPA)的雌激素样活性 ,初步探讨其作用机制。方法 :未成年SD雌性大鼠 (2 1d) 10 0只 ,分为BPA 2 0 0mg/kg、4 0 0mg/kg和 80 0mg/kg 3个剂量组 ,溶剂对照组 (花生油 )和阳性对照组 (苯甲酸雌二醇 ,EstradiolBenzoate,E2 B) 0 .4mg/kg ,每天一次灌胃给药 ,连续给予 3d和 7d。于末次给药的第 2 4h处死动物取子宫。结果 :3d和 7d两个时段 ,E2 B、BPA 4 0 0mg/kg和 80 0mg/kg 2个剂量组子宫湿重、子宫 /体重比、平滑肌厚度和宫腔上皮高度与溶剂对照组相比有显著性差异 (P <0 .0 1) ,有明显的剂量效应关系。给予BPA 7d还使未成年SD雌性大鼠阴道开口时间提前。结论 :BPA具有弱雌激素样效应 ,子宫湿重增加可能与平滑肌增生有关。

Purpose: Uterotrophic assay has been kown efficient for detecting endocrine disrupters. To examine the estrogenic activity of bisphenol A(BPA) using the immature female SD rat. Methods: The vehicle control(the peanut oil), positive control(estradiol benzoate, E\-2B, 0.4 mg/kg )and BPA(200 mg/kg,400 mg/kg and 800 mg/kg) were given orally for 3 and 7 consecutive days beginning postnatal day 21. Then rats were sacrificed 24 hour after the last administration. Results: Uterine weight, uterine/weight, the thickness of the myometrium and the height of the luminal epithelium increased in E\-2B, BPA 400 mg/kg and 800 mg/kg group(P<\{0.01)\} with a dose response relationship. Administered BPA for 7 days, the vaginal opening time of the immature female SD rats advanced.Conclusion: BPA has weak estrogenic activity in SD rat. The hypertrophy and hyperplasia of myometrial cell of uterus may induce the uterine weight increase.