高效液相色谱法测定地锦草中槲皮素的含量

Determination of Quercetin in the Herba Euphorbiae Humifusae

目的 :建立地锦草中槲皮素高效液相色谱测定方法。方法 :色谱柱Kromasil C18(4.6mm× 2 5 0mm ,5μm) ,流动相为乙腈 0 .4 %磷酸 (40∶6 0 ) ,检测波长 370nm ,流速 1.0mL·min-1。结果 :槲皮素在 0 .0 36 4～ 0 .12 74μg线性关系良好 (r =0 .9999) ,回收率 10 0 .7% ,RSD 2 .5 %。 结论 :为地锦草及其制剂提供了分析方法 ,该法简便 ,结果可靠。

Objective: To develop an HPLC method for determining quercetin in Herba Euphorbiae Humifusae. Method: ODS column was used with acetonitrile: 0.4% phosphoric acid (40∶60) as mobile phase. The detection wavelength was at 370 nm and the flow rate was 1.0 mL·min -1. Result: The linearity was obtained over the range of 0.0364～0.1274 μg (r=0.9999). The average recovery was 100.71% with relative standard deviation of 2.45%. Conclusion: This method can be used for quality control of Herba Euphorbiae Humifusae. It is simple, rapid and reliable.