血清白细胞介素-8与溃疡性结肠炎的关系

Relationship between Serum Interleukin-8 and Ulcerative Colitis

背景:炎症细胞因子在溃疡性结肠炎（UC）的发病中可能起一定作用。目的:检测UC患者的血清白细胞介素（IL）-8含量,并分析其与UC病变范围、病变程度和复发与否的关系。方法:收集64例经内镜检查证实的UC患者的血清标本,用酶联免疫吸附测定（ELISA）检测IL-8含量,并与正常对照组进口比较。结果:UC患者的血清IL-8含量为685pg/ml±790pg/ml,正常对照组为25pg/ml±21pg/ml（P<0.000）。不同病变范围UC的血清IL-8含量分别为:愈合期病变289pg/ml±373pg/ml,直肠病变499pg/ml±736pg/ml,直乙状结肠病变686pg/ml±755pg/ml,左半结肠病变 1407pg/ml±846pg/ml,全结肠病变815 pg/ml±926pg/ml;左半结肠病变者的IL-8含量最高,与愈合期和直肠病变者相比有显著差异（P<0.01）。不同病咎程度UC的血清IL-8含量分别为:0级267pg/ml±364pg/ml,1级332pg/ml±418pg/ml,2级999pg/ml±943pg/ml,3级894pg/ml±851pg/ml;2级和3级病变者的含量较0级和1级病变者明显增高（P<0.05和P<0.01）,0级者的含量亦高于正常对照组（P<0.01）。初发患者的血清IL-8含量为758pg/ml±833pg/ml,与复发患者（696pg/ml±803pg/ml）相比无显著差异（p=0.77）。19例患者在正规5-氨基水杨酸（5-ASA）制剂治疗前后分别测定了血清IL-8含量,治疗前的IL-8含?

Background: Inflammatory cytokines may be involved in the pathogenesis of ulcerative colitis (UC). Aims: To measure the serum level of interleukin (IL)-8 in patients with UC and explore the rela-tionship between IL-8 and the extent of lesion and the degree of disease. Methods: The serum of 64 pa-tients with UC proven by endoscopy was collected. The level of IL-8 was measured with enzyme-linked immunosorbent assay (ELISA), and compared with the normal controls. Results: The average level of serum IL-8 in patients with UC was 685 pg/ml±790 pg/ml, and that of normal controls was 25 pg/ml±21 pg/ml (P< 0.000 1). The serum level of IL-8 in different lesions was as follows: in healing stage 289 pg/ml±373 pg/ml, rectum lesions 499 pg/ml±736 pg/ml, rectum and sigmoid lesions 686 pg/ml-±755 pg/ ml, left colon lesions 1 407 pg/ml±846 pg/ml, whole colon lesions 815 pg/ml±926 pg/ml. The serum level of IL-8 in patients with left colon lesions was higher than that in patients with healing stage and rectum lesions (P<0.01). The serum level of IL-8 was 267 pg ml±364 pg/ml in degree 0, 332 pg/ml±418 pg/ml in degree one, 999 pg/ml±943 pg/ml in degree two, and 894 pg/ml±851 pg/ml in degree three. The level of IL-8 was higher in degree 2 and 3 colitis than those of in degree 0 and 1 colitis (P<0.05 and P<0.01). The serum level of IL-8 in patients with degree 0 colitis was higher than that of normal controls (P<0.01). The serum level of IL-8 in patients with first attack was 758 pg/ml±833 pg/ml, and 696 pg/ml±803 pg/ml with recurrent attacks (P=0.77). There were 19 patients received routine treat-ment with 5-aminosalicylic acid (5-ASA). Before treatment the serum level of IL-8 was 983 pg/ml ±737 pg/ml, and it decreased to 342 pg/ml±433 pg/ml after treatment (P= 0.000 2). Conclusions: The serum level of IL-8 in patients with UC is higher than that of normal controls. There is a trend that the more the extensive mucosal damage, the higher the serum level of IL-8. After treatment with 5-ASA, the se-rum level of IL-8 decreases significantly.