c-myc、bcl-2与细胞凋亡在人类皮肤肿瘤中表达及意义

Expression of c-myc, bcl-2 and Apoptosis in the Human Cutaneous Squamous Cell Carcinoma

目的 通过研究皮肤肿瘤中c -myc、bc1- 2蛋白与细胞凋亡的表达 ,探讨其在皮肤组织恶性转化进程中的临床病理意义。方法 采用三羟基末端标记法 (TUNEL)和免疫组化 (SABC)技术原位观察 80例皮肤癌中细胞凋亡与c -myc ,bcl- 2蛋白的表达 ,以 10例正常组织作对照。结果 c -myc蛋白在皮肤癌中阳性率为6 2 5 %,高于正常组织 (P <0 0 5 ) ;bcl- 2蛋白在皮肤癌中阳性率分别为 38 8%,低于正常组织 (P <0 0 5 ) ;细胞凋亡广泛存在于皮肤鳞癌中和正常组织中 ,皮肤鳞癌中细胞凋亡明显高于正常组织 (P <0 0 5 )。结论 皮肤鳞癌中c -myc可能有促进细胞凋亡的作用 ,bc1- 2可能有抑制细胞凋亡的作用。

Objective To explore the clinicophathological significance of expressions of c-myc,bcl-1 and apoptosis in the human cutaneous squamous cell carcinoma(SCC) canceration course.Methods The expressions of c-myc proteins,bcl-2 protein and apoptosis were detected in 80 cases of SCC by using TdT-mediated dUTP-biotin nick end labeling and immunohistochemical staining.Results The positive rates of c-myc and bc1-2 proteins were 62.5%,38.8% respectively,in SCC tissue(P<0.05).Apoptosis widely existed in the normal tissue and SCC tissue.The expression rate of apoptosis in SCC tissue was significantly higher than that of normal tissue(P<0.05).Conclusions The c-myc may be play a promoting role in apoptosis of SCC,the bcl-2 may be play a suppressive role in apoptosis of SCC.