纤维结合蛋白和枸橼酸处理牙根面对人牙龈成纤维细胞功能的影响的实验研究

The Influence of the Application of Fibronection and/or Citric Acid to Root Surfaces on the Migration,Attachment and Orientation of Human Gingival Fibroblasts in Vitro

用体外组织细胞培养方法,采用相差显微镜、微机光笔图象分析系统等手段测算人牙龈成纤维细胞(HGF)对单独使用纤维结合蛋白(fibronectin,Fn)或枸橼酸以及二者联合处理牙根的附着指数(AI)和定向生长指数(OI)。结果表明:(1)单独使用 Fn 处理牙根组的 AI 和 OI 均高于牙根不作任何处理的对照组(P<0.01);(2)单独使用枸橼酸处理牙根组,仅使 OI 高于对照组(P<0.01);Fn 应用于首先用枸橼酸处理的牙根不仅使 AI 高于对照组(P<0.01),且 OI 高于单独使用 Fn 处理牙根组(P<0.05)。

The objective of this study was to evaluate the influence of four different root surfaces:(1)citric acid(CA)conditioned root surface,(2)Fn-treated root surface,(3)CA/Fn treated root surface,(4)non-treated root surface,on the migration,attac-hment and orientation of human gingival fibroblasts(HGF)in vitro.The results indicatethat Fn is an effective biological substance in enhancing attachment and growth offibroblasts on the root surface and the most effective method in this respect may beprovided by CA/Fn conditioning root surface.