大鼠睾丸Sertoli细胞外质特化结构生后发育...

ULTRASTRUCTURAL FEATURES OF THE POSTNATAL DEVELOPMENT OF THE ECTOPLASMIC SPECIALIZATION IN RAT SERTOLI CELLS

本研究采用透射电镜法,对生后1～8周和成年期大鼠睾丸Sertoli细胞外质特化结构(ES)的发育过程进行观察。结果表明,生后第1周ES处于初建阶段,质膜下见高电子密度物质聚集,并见有间断排列的内质网短池。第2周时微丝束开始出现,沿质膜下伸展,内质网池逐渐融合。第3周时微丝密度增加;相邻细胞膜间开始出现紧密连接。随生长发育ES亦逐渐完善,至生后第5周时已于Sertoli细胞基底部周缘广泛伸展。此后直至成年,其形态结构和分布未见有显著改变。本研究认为,大鼠在出生后5周内是ES的重要发育期,与血-睾屏障的形成和完善相一致。

The postnatal development of the ectoplasmic specialization (ES) of Sertoli cells was investigated in the rats from 1st to 8th week after birth and the adult by electron microscopy. At the end of the 1st week. ES is in the beginning of its formation. Some high electron dense materials accumulate in the submembranous regions and short cisternae of endoplasmic reticulum align in these zones. At the end of the 2nd week the short cisternae are fused each other and straight microfilaments bundles appear and are sandwiched between the flattened cisternae and cell membrane. The density of microfilaments is increased in the 3th week and numerous tight junctions appear between adjacent Sertoli cells. The ES is gradually completed in its structure until the 5th week and fully extend and circumscribed around the base of Sertoli cells. Thereafter, there is no more morphological changes. We conclude that the first 5 weeks after birth is the important period for the development of ES of Sertoli cell as well as the blood-testis barrier in rats.