rhIL-2/GM-CSF融合蛋白抗体制备、鉴定及其生物学活性研究

Preparation, identification and biological activity of rhIL-2/GM-CSF fusion protein antibodies

目的 :制备 rh IL - 2 / GM- CSF融合蛋白的抗体 ,并观察该抗体的特异性及对融合蛋白生物学活性的影响。方法 :用纯化的融合蛋白 (rh IL - 2 / GM- CSF)作为抗原 ,免疫新西兰兔后制备抗血清 ,用 EL ISA和 Dot- EL ISA检测抗体滴度和特异性。用依赖株细胞增殖实验检测抗体对 rh IL - 2 / GM- CSF生物学活性的影响。 结果 :此抗体效价高 ,特异性好 ,不仅与融合蛋白(rh IL - 2 / GM- CSF)、IL - 2以及 GM- CSF发生反应 ,而且能够竞争抑制 rh IL - 2 / GM- CSF的生物学活性。 结论 :此抗体可用于融合蛋白 (rh IL - 2 / GM- CSF)

Objective: To prepare and identify recombinant human IL 2/GM CSF(rhIL 2/GM CSF) fusion protein antibodies and to study its specificity and its effect on fusion protein biological activity. Methods: rhIL 2 /GM CSF fusion protein was purified by DEAE Sepharose FF ion exchange chromatography. The purified protein was used to immunize rabbits for the preparation of antisera. The titer and specificity of the antisera were detected by ELISA and Dot ELISA and the biological activity by cell proliferation. Results: The antisera not only reacted with the rhIL 2/GM CSF, IL 2 and GM CSF, but also inhibited the biological activity of the rhIL 2/GM CSF, IL 2 and GM CSF. Conclusion: The obtained antisera can be used to study the structure and function of the rhIL 2/GM CSF.