摘要
A new culture medium for the synthesis of exopolysaccharide by streptococcus salivarius subsp.thermophilus was formulated,based on MRS,M17,ATP,SL,Elliker,SDM usually used in lactic acid bacterium.The suitability of a semi defined culture media (named LCX) was obtained.The formula of culture medium was optimized as:peptone,1.50%,typtone,1.00%;K 2HPO 4,0.20%;MgSO 4.7H 2O,0.02%;MnSO 4.4H 2O,50 mg·L -1 ;Tween 80,1ml·L -1 ;Sodium acetate,0.50%;Sodium glycerate,1.90%.
A new culture medium for the synthesis of exopolysaccharide by streptococcus salivarius subsp.thermophilus was formulated,based on MRS,M17,ATP,SL,Elliker,SDM usually used in lactic acid bacterium.The suitability of a semi defined culture media (named LCX) was obtained.The formula of culture medium was optimized as:peptone,1.50%,typtone,1.00%;K 2HPO 4,0.20%;MgSO 4.7H 2O,0.02%;MnSO 4.4H 2O,50 mg·L -1 ;Tween 80,1ml·L -1 ;Sodium acetate,0.50%;Sodium glycerate,1.90%.
