大白菜3号、6号染色体双三体及其初级三体的鉴定

Identification of Double Triplo-3,6 and Acquisition of Primary Triplo-3 and Triplo-6 in Chinese Cabbage

利用有丝分裂染色体核型分析的方法 ,鉴定同源四倍体大白菜小孢子株系 940 5 1为 3号、 6号染色体双三体 ;在其与冠 2 91杂交的F1代中 ,利用减数分裂中期Ⅰ染色体观察的方法 ,鉴定出了 3号染色体三体和 6号染色体三体 ,并对F1代中的两种三体及双三体的数目进行统计 ,计算出双三体中的 3号、 6号额外染色体的雌配子传递率分别为 2 8.2 6 %和 47.83%;利用TTC染色方法测定 3号染色体三体、 6号染色体三体及双三体的花粉生活力 ,结果表明 :3号染色体三体、 6号染色体三体及双三体的花粉生活力差异达极显著水平。

An autotetraploid Chinese cabbage〔Brassica campestris ssp.pekinensis(Rupr.)Olsson〕 microspore line 9405-1 was designated as double triplo-3,6 by mitotic chromosome karyotype analysis.By meiosis metaphaseⅠ chromosome observation,triplo-3,triplo-6 and double triplo-3,6 were identified in the F 1 of line 9405-1×Guan 291.The transmission of extra chromosomes in double triplo-3,6 was calculated and the result showed that the rates of No.3 and No.6 chromosome were 28.26% and 47.83% respectively.The pollens of triplo-3, triplo-6 and double triplo-3,6 were stained with TTC and their activities were measured.The result showed that the differences of pollen activity among triplo-3,triplo-6 and double triplo-3,6 were significant at 0.01 level.