愈伤组织辐射诱变选育玉兰新品系

Selective Breeding of a New Breed of Magnolia denudata from Radiation-induced Mutation of Callus

玉兰茎尖生长点在MS +环烷酸钠 0 .1mg/L +6 BA 1.5mg/L +2 ,4 D 1mg/L +蔗糖 45g/L培养基上诱导的愈伤组织 ,进行 2 .475× 10 -10 C/kgγ射线辐射处理后 ,接种在MS +ZT 2mg/L +2 ,4 D 1mg/L培养基上 ,分化出具有多种变异性状的不定苗。对具有优良性状的不定苗进行生根培养并移栽 ,从中选出一株一部分生长旺盛、另一部分与母株基本一样的嵌合体植株。对嵌合体具有优良性状的突变部分进行无性繁殖 ,选育出具有一年两次开花、生长速度快而旺盛、抗逆性强等多种优良性状的玉兰新品系。

C/kg γ ray was used to radiate the callus of Magnolia denudata induced from the growing-point in the medium MS+Sodium naphenate 0.1mg/L+6-BA 1.5mg/L+2,4-D 1mg/L+sugar 45g/L.In the medium MS+ZT 2mg/L+2,4-D 1mg/L,adventitious seedlings with variation properties can be differentiated from some treated tissues.Those good seedlings were transplanted into soil and among them a chimaera with one part growing well and the other part basically same as the original parent was selected.A sexual reproduction was carried out on the mutation part with good variation characters.As a result,new breed bearing qualities such as growing fast and luxuriantly having strong resistance,blooming twice a year etc.was obtained.