桃幼胚离体培养再生植株的研究

Plant Regeneration from Excised Immaturate Embryos of Peach(Prunus persica L.)

以中晚熟桃品种‘京艳’、‘绿化 9号’、‘晚蜜’和‘大久保’为试材 ,初步建立了桃体细胞胚发生和增殖的三阶段程序 ,即胚性愈伤组织诱导、体细胞胚发生和发育、体胚萌发成株以及次级体细胞胚的增殖。各品种体细胞胚的萌发成株率分别为 73.5 %、 38.6 %、 5 .4%和 5 8.2 %。用苯脲类细胞分裂素TDZ有效诱导‘京艳’的幼胚子叶直接再生植株 ,再生率 70 %,平均每外植体不定芽数 13.8个 ,再生不定芽生根后获得完整植株。

A three-stage culture program of somatic embryogenesis,consist of induction of embryogenic callus, formation and development of somatic embryos,regeneration of intact plantlets,was developed in in four peach(Prunus persica)cultivars,'Jingyan','Lühua 9','Wanmi'and'Dajiubao'.The percentage of germination and plant regeneration from normally developed soamtic embryos in the cultivars was 73.5%,38.6%,5.4% and 58.2%,respectively.TDZ is effective in stimulating adventitious bud regeneration from immature cotyledons of'Jingyan',70% differentiation rate and 13.8 adventitious buds per cotyledon were obtained.The regenerated adventitious shoots rooted at a rate of 90%.