缺血再灌注对兔心肌肌浆网Ryanodine受体2功能的影响

Effect of ischemia-reperfusion on ryanodine receptor 2 of rabbit cardiac sarcoplasmic reticulum

目的 评价缺血再灌注 (I/ R)对心肌肌浆网 (SR)钙释放功能的影响 .方法 将 16只新西兰兔完全随机平分为对照组、缺血再灌注组 ;采用 langendorff离体心脏灌注模型 ;蔗糖密度梯度离心法制备心肌肌浆网 ;微孔滤膜过滤技术测定心肌肌浆网钙转运功能改变 ;半定量 RT- PCR检测相应蛋白质 m RNA表达水平改变 .结果 与对照组相比 ,缺血再灌组肌浆网摄钙增幅未见减少 ,而 Ryanodine受体 2 (RYR2 )m RNA表达水平明显减少 .结论 缺血再灌注后心肌肌浆网Ryanodine受体 2基因表达减少 ,然其总的释钙量未见减少 .

AIM To assess the alterations of ryanodine receptor 2(RYR2) function and gene expression in ischemia reperfused (I/R) rabbit cardiac sarcoplasmic reticulum (SR). METHEDS Sixteen rabbits were averagely divided into two groups of control, ischchemia reperfusion; the samples were obtained by the langendorff isolated hearts mode; Cardiac sarcoplasmic reticulum membranes isolated by discontinuous sucrose centrifugation were used for assays of SR Ca 2+ transport activities; Using semi quantative reverse transcription polymerase chain reaction (RT PCR), the alteration of ryanodinc receptor 2 mRNA expression level in cardiac SR was detected. RESULTS The increment of Ca 2+ uptake in I/R group was not lower than that in control group,and ryanodinc receptor 2 mRNA expression level in I/R group got lower compared with that in control group. CONCLUSION After ischchemia reperfusion, cardiac SR ryanodinc receptor 2 mRNA expression decreased, but the total Ca 2+ release was not reduced.