大鼠骨髓间充质干细胞向心肌细胞的体外诱导分化

In vitro induction of rat meseachymal stem cells into cardiomyocytes

目的 通过体外药物诱导骨髓间充质干细胞(MSCs)向心肌样细胞分化 ,进一步验证其多能分化特性 .方法 分离培养大鼠 MSCs,5 -脱氧杂氮胞苷 (5 - Aza- cd R)作用2 4 h后继续培养 4 wk,电镜超微结构观察 ,免疫细胞化学染色鉴定 .结果 5 - Aza- cd R诱导 4 wk后的部分大鼠骨髓间充质干细胞 α- Actinin,Troponin T阳性表达 ,电镜观察可见横纹样结构形成 ,核呈卵圆形 ,位于细胞中央位置 ,胞质中可见富集的糖原颗粒及大量线粒体 .结论 MSCs是存在于骨髓中的多能干细胞 ,能在体外条件下经 5 - Aza- cd

AIM To further substantiate the multipotentiality of mesenchymal stem cells (MSCs),we have MSCs induced into cardiomyocytes by 5 Aza 2 deoxycytidine (5 Aza cdR) in vitro. METHODS Rat MSCs were isolated and cultured, then induced by 5 Aza cdR for 24 h. After cultured for additional 4 weeks, MSCs were identified by means of transmission electron microscopy technique and immunochemistrytechnique. RESULTS Some of the MSCs were positive expression of α Actin, Troponin T. Electron microscopy revealed typical striation, centrally positioned neuclei, Rich glycogen granules and a number of mitochondria. CONCLUSION MSCs are mutipotential stem cells which exit in the bone marrow, and they can be induced into cardiomyocytes by 5 Aza cdR in vitro .