羟磷灰石涂层钛合金植入材料的实验研究

Experimental study of the implant of hydroxyapatite-coated titanium alloy

目的 通过测定一种新的羟磷灰石 (HAP)涂层钛合金植入材料的理化参数、与宿主骨的生物相容性观察及骨 HAP界面最大剪切强度的测定 ,评估该材料作为人工骨或人工关节材料的临床应用价值。方法 ①测定材料的最佳烧结温度和机械强度 ,观察材料表面的多孔结构。②取 2 4只新西兰种家兔 ,在两侧股骨外髁上共植入 4 8个HAP表面涂层及非涂层钛合金材料 ,分别于术后 1、2、3、6及 12周处死家兔取材。测定骨 HAP界面最大剪切强度并作组织学观察。结果 ①材料的最佳烧结温度为 80 0°C ,抗剪强度为 18.5MPa ,抗压强度为 170MPa ,孔隙率为 10 %～ 15 % ,孔径为 30～ 70 μm。②HAP涂层钛合金组能与宿主骨紧贴 ,新骨能迅速长入材料的裂隙和被降解的封闭小孔内 ,而非涂层材料则使新生骨组织死亡并为结缔组织取代。③术后任一时间段涂层材料骨 HAP界面最大剪切强度均明显高于非涂层组。

Objective The physical and chemical parameters of a new implant of hydroxyapatite coated titanium alloy, its biocompatibility to host bone and the maximal shear strength at the interface between bone and HAP were investigated, with simultaneously assess the value of this product as artificial bone or prosthetic material in clinical application. Methods ①The best sintered temperature, the mechanical strength and porous structure of this implant were tested. ②48 plugs of HAP surface coated or uncoated titanium alloy were implanted in the lateral femoral condyles of 24 New Zealand rabbits. The animals were killed and the plugs were removed at 1,2,3,6 and 12 weeks postoperatively. The maximal shear strength at the interface between bone and HAP were tested, and the specimens were examined histologically.Results ①The best sintered temperature of this product was 800°C , shear strength was 18.5 MPa and pressure strength 170 MPa, the porosity rate was 10%～15%, the pore size was 30～70 μm. ②The HAP coated titanium alloy could be in close contact with the host bone resulting of new bone formation in the gaps of the material and in the closed pores formed by the degradation of the material. ③The maximal shear strength in the HAP coated group at any postoperative period was higher than that in the uncoated group. Conclusion This implant can be applied clinically as an artificial bone or prosthetic material.