四种单味中药及其复方对小鼠血吸虫病肝纤维化Ⅰ、Ⅲ型胶原及TGF-β1表达的影响

Effects of single use and integrated use of four herbs on the expression of Type Ⅰ/Ⅲ collagen and TGF-β1 in schistosomiasis japonica mice with liver fibrosis

目的研究当归、黄芪、丹参和赤芍四种单味中药及其复方对日本血吸虫病早期肝纤维化的小鼠肝组织Ⅰ型胶原α1(Ⅰ)链COL1A1、Ⅲ型胶原α1(Ⅲ)链COL3A1 及转化生长因子β1 (TGF-β1)mRNA 表达的影响。方法建立日本血吸虫病肝纤维化小鼠模型，随机分为6 组：A 组（当归组），B 组（黄芪组），C 组（丹参组），D 组（赤芍组），E 组（当归、黄芪、丹参、赤芍复方组）和F 组（吡喹酮组），其中F 组用吡喹酮单独治疗，其他各组先用吡喹酮治疗后再用中药治疗，同时设有N 组对照，各组内按照治疗期长短又分为1 组（20 天组）和2 组（40 天组）。治疗结束后应用实时荧光定量PCR（Real-time PCR）的方法检测小鼠肝组织中COL1A1、COL3A1 及TGF-β1 mRNA 的表达。结果A、B、C、D、E 组COL1A1、COL3A1 及TGF- β1 mRNA 的表达明显低于F 组，P<0.001；E 组的表达量最低，E1 组的COL1A1、COL3A1 及TGF-β1 mRNA 的表达显著低于A1、B1、C1、D1 组，P<0.05～0.001；E2 组COL1A1 表达明显低于A2、B2、D2，P<0.05，E2 组COL3A1 和TGF-β1 的表达明显低于B2、D2 组，P<0.05。结论单味中药当归、黄芪、丹参、赤芍及其复方均有抗血吸虫病肝纤维化的作用，能显著降低Ⅰ、Ⅲ型胶原的生成，抑制TGF-β1 的表达，复方比单方能更好更快的发挥疗效。

Objectives To examine the effects by single use and integrated use of the four herbs Angelicasinensis, Astragalus mongholicus, Salvia miltiorrhiza, Radix Paeoniae Rubra on mRNA expression of type I collagenα1 (Ⅰ) chain (COL1A1)gene, type Ⅲ collagen α1(Ⅲ) chain(COL3A1)gene and TGF-β1 gene in liver tis?sues of hepatic fibrosis mice due to schistosoma japonicum infection. Methods Liver fibrosis models from schisto?somiasis were developed in mice and then randomized into group A(treated with Angelica sinensis), B(treatedwith Astragalus mongholicus), C(Salvia miltiorrhiza), D(Radix Paeoniae RubraⅡ), E(integrated treatment with thefour herbs) and F(treated with praziquantel). Group F received simple praziquantel, and remaining groups weregiven additional herbal components besides praziquantel. Group N was used as controls, the six groups weresubgrouped into two by the treatment course(20 days and 40 days). After treatment, Real-time PCR was per?formed to determine the mRNA of COL1A1, COL3A1 and TGF-β1. Results Group A, B, C, D and E hadbetter treatment effects on reduction of the mRNA expression of COL1A1, COL3A1 and TGF-β1 comparedto group F group(P<0.001). Among treatment group, mRNA expression of COL1A1, COL3A1 and TGF-β1 in group E1 was significantly lower than that of group A1, B1, C1and D1(P<0.05 or P<0.001).Group E2had lower mRNA expression of COL1A1 than group A2, B2 and D2(P<0.05), and expression of COL3A1and TGF-β1 was lower than that of group B2 and D2(P<0.05). Conclusion Either single use or integrated use of the four herbs Angelica sinensis, Astragalus mongholicus, Salvia miltiorrhiza, Radix Paeoniae Rubra can im?prove the liver fibrosis from schistosomiasis and significantly downregulate mRNA expression of COL1A1,COL3A1 and TGF-β1. However, integrated use of the four herbs may produce better and faster effects thansingle recipe .