甜椒花药培养及再生植株技术体系的研究

In vitro Culture and Plant Regeneration Technology System of Capsicum annuum var. grossum Anthers

为建立甜椒(Capsicum annuum var.grossum)花药培养及再生植株技术体系,对影响花药胚状体诱导和分化的因素进行了研究。结果表明,培养基组成对胚状体诱导率的影响以NAA>基本培养基>椰乳>KT,最佳胚状体诱导培养基为NTH+0.1 mg L–1 NAA+10%椰乳+1 mg L–1 KT+50μmol L–1 Ag NO3+30 g L–1蔗糖+5 g L–1琼脂+2 g L–1活性炭。花药经过24 h低温预处理和8 d高温预培养后,胚状体诱导率可达23.38%。植物生长调节剂对胚状体出芽率的影响为6-BA>NAA>IAA,最佳胚状体分化培养基为NTH+1 mg L–1 6-BA+0.3 mg L–1 NAA+0.1 mg L–1 IAA+30 g L–1蔗糖+5 g L–1琼脂。胚芽转入1/2MS+0.5 mg L–1 IBA+30 g L–1蔗糖+5 g L–1琼脂培养基后,生根率可达92.5%。

The aim was to establish plant regeneration system from anthers of Capsicum annuum var. grossum.The influence factors on embryo induction and differentiation were studied. The results showed that the influencesof medium compositions on embryo induction were in the order of NAA>basal medium>coconut milk>KT.The optimum medium for embryo induction was NTH + 0.1 mg L–1 NAA + 10% coconut milk + 1 mg L–1 KT +50 μmol L–1 AgNO3 + 30 g L–1 sugar + 5 g L–1 agar + 2 g L–1 activated carbon. After pretreated for 24 h in lowtemperature and precultured for 8 d at high temperature, embryo induction rate could reach 23.38%. The effect ofplant growth regulator on bud rate from embryo was in the order of 6-BA>NAA>IAA, and the optimum mediumfor embryo differentiation was NTH + 1 mg L–1 6-BA + 0.3 mg L–1 NAA + 0.1 mg L–1 IAA + 30 g L–1 sugar + 5 g L–1agar. Then, embryo buds were cultured on 1/2MS + 0.5 mg L–1 IBA + 30 g L–1 sugar + 5 g L–1 agar medium,rooting rate could reach 92.5%.