摘要
目的研究胶质瘤中骨桥蛋白(OPN)、半胱氨酸天冬氨酸蛋白酶-3(caspase-3)和突变型P53(mt-P53)蛋白的表达意义和相关性。方法选取脑胶质瘤(胶质瘤组)患者的标本70例,非脑胶质瘤脑组织10例作为对照组。应用免疫组织化学SP法检测2组OPN、caspase-3及mt-P53蛋白的阳性表达率。蛋白免疫印迹法检测脑胶质瘤中OPN、caspase-3和mt-P53蛋白表达与脑胶质瘤级别的关系。Spearman等级相关分析OPN、caspase-3和mt-P53表达阳性的关系。结果脑胶质瘤组OPN、mt-P53阳性表达率(64.29%和60%)均高于对照组(无阳性表达),caspase-3阳性率低于对照组(47.14%vs.90%),差异有统计学意义(均P<0.05)。OPN、caspase-3和mt-P53在脑胶质瘤不同性别、年龄、肿瘤大小及肿瘤部位组间的阳性表达率差异无统计学意义(P>0.05)。胶质瘤分级越高,OPN和mt-P53的阳性表达率越高,而caspase-3的阳性表达率越低(均P<0.001)。随着脑胶质瘤级别的增加,OPN和mt-P53蛋白表达水平上调,而caspase-3的蛋白表达水平下降。OPN与caspase-3阳性表达呈负相关,与mt-P53表达呈正相关(rs分别为-0.720和0.722,P<0.05),caspase-3和mt-P53表达呈负相关(rs=-0.556,P<0.05)。结论胶质瘤分级越高,OPN和mt-P53的阳性表达率越高,而caspase-3的阳性表达率越低;三者与胶质瘤发生、发展均密切相关,联合检测有助于对胶质瘤生物学行为的判断。
Objective To study the expressions of osteopontin (OPN),caspase-3 and mt-P53 proteins, and their relationship in gliomas. Methods Seventy gliomas specimens of patients (glioma group) were selected, and 10 samples of non-glioma brain tissue were used as control group. The SP method was used to detect the positive rates of protein expressions of OPN, caspase-3 and mt-P53 between two groups. The relationship between protein expressions of OPN, caspase-3 and mt-P53 in gliomas and grade of gliomas were detected by Western blot assay. Spearman rank correlation was compared between the positive expression of OPN, caspase-3 and rate mt-P53. Results The positive expression rates of OPN and mt-P53 were significantly higher in glioma group (64.29% and 60%) than those of control group (no positive expression), but the positive expression rate of caspase-3 was significantly lower than that of control group (47.14% vs. 90%, P<0.05). There were no significant differences in OPN,caspase-3 and mt-P53 expressions between different gender, age, tumor size and tumor position (P > 0.05). The higher the WHO classification, the higher the positive expression rates of OPN and mt-P53 (P < 0.001), and the lower the positive expression rate of caspase-3 (P < 0.001). With the increased level of glioma grade, OPN and mt-P53 protein levels were increased, but caspase-3 protein expression level was decreased. There was a negatively correlation between OPN and the positive expression of caspase-3, but there was a positive correlation between OPN and the expression of mt-P53 (rs = -0.720 and 0.722, P < 0.05). There was a negative correlation between caspase-3 and mt-P53 expressions (rs = -0.556, P < 0.05). Conclusion The higher the WHO classification, the higher the positive expression rates of OPN and mt-P53, while the lower the positive expression rate of caspase-3. The study reveals that OPN, caspase-3 and mt-P53 expressions are associated with the occurrence and the progress of gliomas.The combined detection of them can contribute to the judgment of biological behavior of gliomas.
作者
郭可盈
曾苏婷
范伟
曹思源
王莹
陆锦标
王东林
GUO Keying;ZENG Suting;FAN Wei;CAO Siyuan;WANG Ying;LU Jinbiao;WANG Donglin(Department of Pathology,School of Medicine, Nantong University, Nantong 226001, China)
出处
《天津医药》
CAS
2016年第8期1005-1009,共5页
Tianjin Medical Journal
基金
国家自然科学基金资助项目(81272789)
南通大学大学生创新训练计划项目(2014091)