呼吸内科常见菌及鲍曼不动杆菌质粒的耐药基因探讨

Discussion of plasmid resistance genes of acinetobacter baumannii and common bacteria in respiratory department

目的:探讨呼吸内科常见菌及鲍曼不动杆菌质粒的耐药性基因。方法:检测常用的抗菌药物对鲍曼不动杆菌的最低抑菌浓度(MIC50),分析AB质粒上的耐药基因。结果:140株致病菌来源于痰液(92.01%),5株致病菌来源于血液(2.98%)。革兰阴性菌118株,革兰阳性菌22株。除IPM外,1株鲍曼不动杆菌对其他14种常用的抗生素均耐药。供体菌的质粒所带的耐药性均传给了受体菌,接合子带有和供体菌相同的3种基因。结论:分析呼吸内科常见致病菌、研究MDRAB质粒携带的耐药基因型有助于指导和治疗呼吸内科感染。

Objective:To investigate the plasmid resistance genes of acinetobacter baumannii and common bacteria in respiratory department.Methods:The minimum inhibitory concentration (MIC50) of common antibacterial agents on acinetobacter baumannii were detected and the plasmid resistance genes of acinetobacter baumannii were analyzed.Results:140 strains of pathogenic bacteria were from sputum (92.01%);5 strains of pathogenic bacteria were from blood (2.98%).118 strains were gram negative bacteria;22 strains were gram positive bacteria.In addition to IPM,1 strain of acinetobacter baumannii were resistant to the other 14 kinds of commonly used antibiotics.The drug resistance of plasmid in pretreated bacterium were passed to the recipient bacterium,and the conjugon had the same three genes as the pretreated bacterium.Conclusion:The analysis the common pathogenic bacteria in respiratory department and the resistant genotypes of plasmid in MDRAB were helpful to the guide and treatment of infection in respiratory department.