EV71-IgM检测在手足口病诊断中的价值

The value of EV71-Ig M detection in diagnosis of hand foot mouth disease

目的:回顾性分析评价应用胶体法检测EV71-Ig M抗体与RT-PCR法检测EV71病毒在手足口病重症病例早期识别中的作用。方法:收治手足口病疑似病例1 252例,分别采用血清胶体金法检测EV71肠道病毒Ig M抗体,RT-PCR法检测患者大便中肠道病毒及肠道病毒71-RNA。结果:胶体金法检测EV71阳性率28.67%;RT-PCR法检测肠道病毒阳性率81.15%,EV71阳性率44.89%。其中金标法检测阳性5例,RT-PCR法检测阴性。结论:EV71胶体金法可作为EV71感染的初筛早期快检,在临床手足口病重症病例早期识别及治疗中具有重要意义。

Objective:To evaluate the role of detecting EV71-IgM antibody by colloidal method and EV71 virus by RT-PCRmethod in the early identification of severe hand-foot-mouth disease by retrospective analysis.Methods:1 252 suspected cases ofhand-foot-mouth disease were selected.The IgM antibody of EV71 intestinal virus was detected by serum colloidal gold method.The intestinal virus in stool of patients and 71-RNA were detected by RT-PCR method.Results:The positive rate of detectingEV71 by colloidal gold method was 28.67% ;the positive rate of detecting intestinal virus by RT-PCR method was 81.15% ;thepositive rate of EV71 was 44.89%;5 cases were detected as positive by colloidal gold method and negative by RT-PCR method.Conclusion:EV71 colloidal gold method can be used as an early screening of EV71 infection,and it has important significance inearly identification and treatment of severe hand-foot-mouth disease.