重组短链脱氢酶LcSDR催化(R)-1-苯基乙醇不对称合成的工艺开发

The Process Development of(R)-1-Phenylethanol Asymmetric Reduction Catalyzed by A Recombinant Short-Chain Dehydrogenase and Reductase LcSDR

采用基因挖掘技术从Lactobacillus composti基因组中筛选到一条编码短链脱氢酶(LcSDR)的序列,该LcSDR能不对称还原苯乙酮(1a)合成(R)-1-苯基乙醇[(R)-1b]。构建了Lc SDR和葡萄糖脱氢酶(EsGDH)双酶偶联催化合成体系,优化了Lc SDR不对称还原1a合成(R)-1b的催化工艺参数。在较佳催化条件下,50 g/L1a转化反应2 h,产物(R)-1b得率93.8%,产物对映体过量值(eep)高于99%,该手性生物合成催化过程的时空产率达到562.8 g/(L·d)。

A short-chain dehydrogenases and reductases (LcSDR) coding sequence lcsdr was screened outfrom Lactobacillus composti genome via gene mining technology. It was found that LcSDR was able toasymmetrically reduce acetophenone(1a) to (R)-1-phenylethanol [(R)-1b]. The LcSDR and glucosedehydrogenase (EsGDH) double-enzyme conjugate catalytic synthesis system was constructed, and theprocess parameters were optimized for catalytic reduction of 1a to (R)-1b in this study. Under the appropriateconditions, 50 g/L 1a was converted to (R)-1b in 2 h with yield of 93.8%, the enantiomeric excess (eep)above 99% and the space time yield of 562.8 g/(L·d).