生物膜处理高含油废水及膜表面微生物群落特性研究

Study on the high concentration oil-containing wastewater by biofilm treatment of P.aeruginosa strain NY3 and the characteristics of microbial community in biofilm

利用单一铜绿假单胞菌NY3生物膜在敞开体系中(非无菌条件下)处理石油废水,结果发现,生物膜可高效率去除高浓度含油废水中的油类物质.在2g/L含油水样中,连续运行25d,石油烃去除率可持续保持约91.1%.停止进水后,曝气可使生物膜活性恢复.自然环境中,配伍于NY3菌生物膜中的微生物主要有芽孢杆菌、假单胞菌和红球菌属,其中在实验室能够培养出来的微生物经16Sr RNA鉴定分别属于红球菌属和芽孢杆菌属,经检测,2株菌均有高的石油烃降解效率.

Single P. aeruginosa NY3 can grow and be immobilized on the surface of polyurethane foam carrier, thebiofilm was used in an open system (non sterile conditions) to treat the oil-containing wastewater. The results showed thatthe biofilm can efficiently removeoil material of high concentrationoily wastewater. After continuously running for 25 d inthe oil-bearing wastewater of 2g/L, the removal rate of petroleum hydrocarbons can be maintained at 91.1%. When theentrance of water was stopped, the bioactivityof the biofilm can be recovered by aeration. Under the natural environment,the bacterial accompanied and survived with NY3 in bacteria biofilmmainly includedBacillus, Pseudomonas andRhodococcus strain. Two cultured strains were isolated and identified through the 16SrRNAsequence, which showed theybelonged toRhodococcusand Bacillus separately, and they were also proved to have the capabilty to efficiently removepetroleum hydrocarbon.