山茱萸多糖对血管性痴呆大鼠海马BDNF和NGF表达的影响

Effects of the cornel officinalis polysaccharide on m RNA expression of hippocampal BDNF and NGF in rats with vascular dementia

目的:研究山茱萸多糖对血管性痴呆模型大鼠海马神经元数量及脑源性神经营养因子(BDNF)和神经生长因子(NGF)m RNA表达的影响。方法:将30只大鼠随机分为假手术组、模型组、山茱萸多糖处理组,每组10只。假手术组与模型组给予生理盐水灌胃,山茱萸多糖处理组给予0.05 g/m L山茱萸多糖灌胃,连续4周。4周后,用HE染色法观察海马神经元数目变化;RT-PCR法检测海马BDNF m RNA、NGFm RNA表达。结果:与假手术组比较,模型组大鼠海马神经元数目明显减少,BDNF m RNA、NG Fm RNA表达明显下降(P<0.05);与模型组比较,山茱萸多糖处理组海马神经元数目明显增多,BDNF m RNA、NGF m RNA表达明显升高(P<0.05)。结论:山茱萸多糖能够通过上调血管性痴呆大鼠海马BDNF m RNA、NGF m RNA表达,增加大鼠海马神经元的数目,防治血管性痴呆。

Objective: To explore the effects of the cornel officinalis polysaccharide on hippocampal neurons and mRNA expression of BDNF and NGF in rats with vascular dementia. Methods: Thirty rats were randomly divided into three groups: the sham operation group,the model group and the cornel officinalis polysaccharide treatment group, with 10 rats in each group. The sham operated group and the model group were lavaged with saline for 4 weeks and the cornel officinalis polysaccharide treatment group were lavaged with 0.05 g/mL cornus officinalis polysaccharide for 4 weeks. After 4 weeks the neuron number in hippocampus was observed by HE staining and mRNA expressions of BDNF and NGF were detected by RT-PCR. Results: Compared with the sham operated group, the neuron number in hippocampus of the model group was significantly less and mRNA expressions of BDNF and NGF were significantly decreased(P<0.05);Compared with the model group,the neuron number in hippocampus was bigger and mRNA expressions of BDNF and NGF were significantly increased in the cornel officinalis polysaccharide treatment group (P < 0.05). Conclusion: The cornel officinalis polysaccharide could treat vascular dementia by increasing neuron numbers and up-regulating mRNA expression of BDNF and NGF in hippocampus.