一株烷烃降解细菌的分离鉴定及其降解特性

Isolation and Identification of an Alkane Degrading Bacterium and its Degradation Characteristics

从天津海域采集的水样中筛选获得一株烷烃降解细菌,经16S r RNA基因鉴定,结合菌株的形态学特征和生理生化特性分析,鉴定为柠檬酸杆菌属(Citrobacter),命名为Citrobacter sp.TUST-S5.利用GC-MS检测出菌株Citrobacter sp.TUST-S5能降解链长C11—C28的烷烃;对C20以上的长链烷烃,降解率为80%,以上,且降解率随碳链长度的增加呈升高的趋势;菌株的生长曲线及降解曲线显示菌株对烷烃的降解率的变化与菌浓度紧密相关;菌株的乳化活性为47.86%,疏水性为38.30%.菌株Citrobacter sp.TUST-S5对中长链烷烃的降解效果显著,乳化作用明显,对于修复海洋石油污染具有重要的作用.

An alkane degrading bacterium was isolated from the water samples collected from Tianjin sea area.The strain was defined as Citrobacter sp.TUST-S5 according to its physiological and biochemical characteristics as well as 16S rRNA phylogenetic tree.Its degradation rate was determined by GC-MS.Results showed that Citrobacter sp.TUST-S5 had the capacity of degradating C11-C28 alkane and its degradation rate of long chain alkane over C20 was more than 80%,.The growth curve and degradation curve of the strain displayed a change of the degradation rate closely related to the concentration of bacteria.Its emulsifying activity and the hydrophobicity were 47.86%, and 38.30%,.Therefore,Citrobacter sp.TUST-S5 has a good ability to degrade C11-C28 alkane,and will play an important role in remedying marine oil pollution.