自体静脉血在不同离心条件下特定生长因子成分测定与分析性研究

Analysis of Component and Content of Given Bioactive Factors of Autologous Platelet Concentrate under Different Centrifugal Condition

目的:探讨不同制取条件下的PRF、CGF中特定生长因子及其含量,定性分析两者宏观性状与微观结构特征,为临床应用PRF、CGF提供理论依据。方法:8位受试者采用PRF和CGF离心机真空负压采血管制取PRF与CGF;以含有抗凝剂的真空负压玻璃试管采集全血作为对照组。应用酶联免疫吸附试验(ELISA)对各组样本中生长因子PDGF-BB、TGF-β1、BMP2进行定性及定量酶标仪检测。结果:(1)各组PRF及CGF样本肉眼观察:外观性状各组间存在一定差别。(2)PRF及CGF样本随机抽样光镜下及电镜下观察,纤维蛋白结构未见明显区别。(3)除对照组外,A、B、C、D组均检测出PDGF-BB和TGF-β1,各组均未检测到BMP-2。结论:四种不同离心条件下制备的PRF、CGF中:(1)均观察到大量富集的血小板和白细胞;(2)基本物理特征相似,测定的部分生长因子的含量存在统计学差异,本实验的结果将有助于指导临床应用。

Objective: To investigate the specific growth factor and its content in different preparation conditions ofPRF,CGF,qualitative analysis of both macroscopic properties and microstructure characteristics,provide a theoretical basis for theclinical application of PRF and CGF.Methods:Eight subjects by PRF and CGF centrifuge vacuum negative pressure producingPRF and CGF; with anticoagulant glass vacuum tube negative pressure collecting whole blood was taken as the control group.The application of enzyme linked immunosorbent assay (ELISA) for each sample in the growth factor PDGF-BB,TGF- β1,BMP2qualitative and quantitative microplate detection.Results:(1) Observed in PRF and CGF samples:there are some differences inthe appearance of the eye between the groups.(2) To observe PRF and CGF sample random sampling under light microscope andelectron microscope,fibrin structure had no obvious difference.(3) Except the control group,A,B,C,D group were detected PDGFBBand TGF-β1 groups,BMP-2 were not detected.Conclusion:Four different centrifugation conditions of preparation of PRF andCGF in:(1) were observed in a large number of enrichment of platelet and leukocyte; (2) the basic physical characteristics aresimilar,the determination of some growth factor content existence difference,the results of this experiment will help to guide theclinical application.