骨保护素对去卵巢大鼠中种植体骨整合的影响

Effects of osteoprotegerin on implant osseointegration in ovariectomized rats

目的探索去卵巢大鼠中骨保护素(osteoprotegerin,OPG)对种植体骨整合的影响。方法大鼠接受双侧卵巢摘除术后12周于胫骨近端植入2颗钛种植钉。将大鼠按每组10只随机分为OPG组与对照组。皮下注射OPG(10 mg/kg)或安慰剂每周3次。8周后收集实验标本进行μCT和组织学以及生物力学分析。结果μCT结果显示,与对照组比较,OPG组中增加了124%的骨体积,167%的骨结合,111%的平均骨小梁数目,92%的平均骨小梁厚度,95%的平均连接密度;降低了64%的平均骨小梁分离度。骨组织形态计量学评价显示OPG组增加160%的骨面积密度和234%骨-种植体接触。生物力学测试提示OPG组种植体的最大推力增加228%。结论全身应用OPG可以改善去卵巢大鼠骨内种植体骨整合和稳定性,其机制可能是通过增加种植体周围的骨量和改善骨小梁微结构。

Objective To investigate the role of osteoprotegerin (OPG) in implant osseointegration in ovariectomized rats. Methods 12 weeks after bilateral ovariectomy, each rat accepted 2 titanium screws in the proximal tibiae.All animals were then randomly divided into 2 groups: control (10 rats) and OPG group (10 rats). Subcutaneous injection of OPG (10 mg/kg) or vehicle was performed 3 times a week. 8 weeks later, tibiae with screws were harvested for μCT,histological and biomechanical analysis. Results Compared to control, OPG increased the percent bone volume by 124%, the percent osseointegration by 167%, the mean trabecular number by 111%, the mean trabecular thickness by 92%, the mean connective density by 95%; and decreased the mean trabecular separation by 64% in μCT analysis.OPG also increased bone area density by 160% and bone?to?implant contact by 234% in histomorphometric evaluation,and increased the maximal push?out force by 228% in biomechanical test. Conclusion Systemic administration of OPG can improve implant osseointegration and fixation in ovariectomized rats, resulting from the increased peri-implant bone mass and improved trabecular microarchitecture.