外源性右旋糖酐酶和氟化钠对粘性放线菌生物膜的影响

Effects of exogenous dextranase and sodium fluoride on Actinomyces viscosus biofilm

目的探讨外源性右旋糖苷酶(dextranase,Dex)和氟化钠(Na F)对粘性放线菌生物膜的影响。方法用结晶紫(crystal violet,CV)染色法测量不同浓度外源性Dex和Na F作用下粘性放线菌生物膜量;用扫描电镜观察不同实验组生物膜的形态和结构。结果外源性Dex和Na F的浓度升高,对粘性放线菌生物膜形成的抑制作用增强;在实验条件下,0.25 U/m L Dex与40/80μg/m L Na F联用对生物膜形成的抑制作用明显强于二者单独作用。结论外源性Dex和Na F能够抑制粘性放线菌生物膜的形成,且在一定条件下,它们对粘性放线菌生物膜的形成表现出协同抑制作用。

Objective To explore the impact of exogenous dextranase (Dex) and sodium fluoride (NaF) on the biofilm formation of Actinomyces viscosus. Methods Crystal violet was used to quantify the biomass of Actinomyces viscosus treated with serial concentrations of Dex and NaF. Scanning electronic microscope was applied to detect the morphology and structure of the biofilms. Results The formation of Actinomyces viscosus biofilm was inhibited when the concentrations of Dex or NaF rised. Compared with the separate use of 0.25 U/mL Dex and 40 or 80 μg/mL NaF, the combined use of these two agents could obviously inhibit the biofilm formation. Conclusion Both separate and combined use of Dex and NaF could inhibit the Actinomyces viscosus biofilm formation, and they displayed synergistic effect under certain conditions.