HPLC测定十全大补丸、补中益气丸、银翘解毒片中甘草酸含量

Content Determination of Glycyrrhizic Acid in Shiquan Dabu Pills, Buzhong Yiqi Pills, Yinqiao Jiedu Tablets by HPLC

目的建立十全大补丸、补中益气丸、银翘解毒片中甘草酸的含量测定方法。方法采用高效液相色谱法测定十全大补丸、补中益气丸、银翘解毒片中甘草酸的含量，采用ZorbaxSBC18色谱柱（150mm×4.6mm，5μm），流动相为甲醇-2.5%醋酸（65∶35)，流速为0.8mL/min，检测波长为254nm。结果甘草酸在0.1803～3.6054μg范围内与峰面积呈良好的线性关系（r＝1），十全大补丸、补中益气丸、银翘解毒片中甘草酸平均回收率分别为99.85%（n＝6）、100.70%（n＝6）、100.81%（n＝6）。结论本试验所建立的方法专属性强、简便，可用于含甘草成分的产品中甘草酸的检测。

Objective To establish content determination method of glycyrrhizic acid in Shiquan Ddabu Pills,Buzhong Yiqi Pills and Yinqiao Jiedu Tablets. Methods HPLC method was used to determine the content of glycyrrhizic acid in the three preparations. Zorbax SB C18 column (150 mm×4.6 mm, 5 μm) was used; Methanol-2.5% Acetic acid (65∶35) was set as the mobile phase at flow rate of 0.8 mL/min; the detection wavelength was 254 nm. Results The linear range of glycyrrhizic acid was in the range of 0.180 3–3.605 4 μg (r=1). The average recovery was 99.85% (n=6) in Shiquan Dabu Pills, 100.70% (n=6) in Buzhong Yiqi Pills, and 100.81% (n=6) in Yinqiao Jiedu Tablets. Conclusion The method is specific, simple and can be used to determine glycyrrhizic acid in the products containting glycyrrhizae.