酚酸类物质对三七幼苗的化感影响

Allelopathic effects of phenolic acids on Panax notoginseng seedlings

该文研究了不同浓度的阿魏酸、对香豆酸、丁香酸、对羟基苯甲酸、香草酸5种酚酸类物质对三七幼苗生长和生理的影响。结果表明:处理后,三七幼苗的苗高、根长、可溶性蛋白质含量、根系活力、CAT以及POD活性均有所降低。其中,阿魏酸各处理组幼苗的苗高及POD活性均显著降低,50、100 mg·L^(-1)的对香豆酸以及100 mg·L^(-1)的香草酸处理组幼苗苗高也分别比对照显著降低16.19%、16.67%和29.29%;对香豆酸、丁香酸以及对羟基苯甲酸各处理组幼苗根长均显著低于对照;香草酸处理组幼苗的根系活力也显著低于对照,且幼苗的CAT活性在10、50、100 mg·L^(-1)丁香酸、对羟基苯甲酸以及香草酸处理下也达到了显著降低水平。此外,1 mg·L^(-1)阿魏酸以及100 mg·L^(-1)香草酸处理组幼苗的叶绿素含量也均显著降低;中高浓度的阿魏酸、对香豆酸、丁香酸、对羟基苯甲酸增加了三七幼苗的MDA含量,而香草酸在0.1、1、10、100 mg·L^(-1)浓度下显著降低幼苗的MDA含量;丁香酸、香草酸、对羟基苯甲酸以及中高浓度的对香豆酸增加了三七幼苗的SOD活性,且香草酸各处理组均达到了显著性水平。综上结果表明,5种酚酸类物质对三七幼苗均具有一定的化感抑制作用,但各酚酸物质的作用方式及强度并不完全一致,阿魏酸的化感影响较大,这为进一步研究三七的化感自毒作用提供了一定的理论参考。

This article studied the effects of ferulic acid, P-coumaric acid, syringic acid, P-hydroxybenzoic acid and vanillic acid at different concentrations on the growth and physiology of Panax notoginseng seedlings. The results showed that shoot height, root length, soluble protein content, root activity, CAT and POD activity of P. notoginseng seedlings decreased. Among them, shoot height and POD activity of seedlings were decreased obviously by ferulic acid; and the shoot height of 50, 100 mg ? L 1 P-coumaric acid and 100 mg ? L 1 vanillic acid were 16.19% , 16.67% and 29.29% dramatically lower than that of the control group. The root length of seedlings were also reduced signifi-cantly by P-coumaric acid, syringic acid and P-hydroxybenzoic acid, and the root activity of vanillic acid were reduced significantly as well,meanwhile the CAT activity of seedlings were decreased by 10, 50,100 mg ? L1 Syringic acid, P-hydroxybenzoic acid and vanillic acid. In addition, the content of chlorophyll of seedlings were also reduced obvious?ly by 1 mg ? L 1 ferulic acid and 100 mg ? L 1 vanillic acid. Furthermore, the content of MDA increased at higher con - centrations of ferulic acid, P coumaric acid, syringic acid and P hydroxybenzoic acid, while it decreased significantly at 0.1, 1, 10 and 100 mg ? L 1 concentrations of vanillic acid. Besides that, the SOD activity of seedlings increased when treated with syringic acid, vanillic acid, P hydroxybenzoic acid and higher concentrations of P coumaric acid, moreover vanillic acid increased them significantly. The results showed that five phenolic acids had some certain allelo pathic inhibitory effects on P. notoginseng seedlings, but the effects wrere not completely consistent. Among them, feru - lic acid had greater influence. These provides some theoretical reference for further research of allelopathic autotoxicity of P. notoginseng.