祛风止痛胶囊对博来霉素诱导的小鼠肺间质纤维化模型的影响及机制探讨

Effects and mechanisms of Qufeng Zhitong capsule on mice model of bleomycin-induced Pulmonary fibrosis

目的初步探讨祛风止痛胶囊对博来霉素导致的小鼠肺纤维化模型的影响及机制。方法将54只C57BL/6小鼠按随机数字表法分3组,即正常组、模型组、祛风组,每组18只。1组作为正常对照,2和3组小鼠使用博来霉素诱导肺纤维化模型。分别对2和3组予以0.9%氯化钠注射溶液、祛风止痛胶囊药液灌胃处理,对照组予等量0.9%氯化钠注射溶液灌胃。然后于第7、14、28天分批处死小鼠,每次每组随机抽取6只小鼠,留取血样测定细胞因子,并取小鼠肺作病理观察。结果 (1)2组、3组小鼠活动减少,进食,饮水情况亦减少,体质量较1组小鼠减轻。(2)光镜下观察1组未见明显的炎症、纤维化等病理变化,仅于大气道和血管壁周围可见少量蓝色的基质胶原纤维;2组博来霉素诱导的肺纤维化模型成功。3组较2组轻,组间病理评分比较差异有统计学意义(P<0.05)。(3)2组、3组血干扰素-γ(IFN-γ)、白介素-6(IL-6)、白介素-17A(IL-17A)水平较1组升高(P<0.05),3组炎性因子IL-6、IFN-γ、TNF-α、IL-17A水平较2组降低(P<0.05)。结论祛风止痛胶囊对博来霉素诱导的小鼠肺纤维化模型的肺泡炎和肺纤维化有抑制作用;祛风止痛胶囊对炎性因子IL-6、IFN-γ、TNF-α、IL-17A有抑制作用。

Objective To investigate the effects and mechanisms of Qufeng Zhitong capsule in mice model of ble?omycin -induced Pulmonary fibrosis. Methods 54 C57Biy6 mice were randomly divided into 3 groups after feeding a?daptively for 1 week,and the normal group was the group numbered 1,model group was 2,and Qufeng group was 3,with 18 mice every group. The mice of group 2-3 were induced into the pulmonary fibrosis model using bleomycin. The mice of group 2-3 were respectively received the same amount of normal saline and the liquid of Qufeng Zhitong capsule with the gavage way. Then on the 7th, 14th and 28th day,6 mice were sacrificed srandomly in each group every time. Collect blood. The blood specimens were used to measure the cytokines and the lung was used for the pathological observation. Results (1)^ mice activity , eating and drinking decreased in group 2 and group 3 and the body weight was also less than the group 1. There was no abnormal situation about activities,eating and drinking of the mice in group 1. (2)There was no sig?nificant pathological changes of inflammation and fibrosis observed under light microscope in group 1, a small amount of blue collagen matrix was showed only around airways and blood vessel wall. In group 2 the mice model of bleomycin-in?duced Pulmonary fibrosis was successful. There were significant differences (P <0. 05) between the group 2 and group 3 about the pathological scores with the score of group 3 less than the group 2. ( 3 ) IL-6, IFN^7,TNF-a, IL-17A levels of group 2 and group 3 were higher than those of the group 1 ,with statistical significance (P <0. 05). IL-6,IFN-7,TNF-a, IL-17A levels of group 3 were lower than those of the group 2 with significant differences (P <0. 05). Conclusions Qufeng Zhitong capsule has a inhibition effect on the alveolitis and pulmonary fibrosis of mice models bleomycin-inducedpulmonary fibrosis,which may be related its inhibition on the cytokine IL-6,IFN^7,TNF-a and IL-17A