桑色素对全氟辛酸诱导小鼠肝损伤的保护作用

Protective Effect of Morin on Perfluorooctanoic Acid-Induced Liver Injury in Mice

目的探讨桑色素(morin,Mor)对全氟辛酸(perfluorooctanoic acid,PFOA)诱导小鼠肝损伤的保护作用。方法将24只雄性小鼠按随机数字表法分为4组:对照组(等容量生理盐水)、PFOA组(PFOA 10 mg·kg^(-1)·d^(-1))、Mor组(Mor 75mg·kg^(-1)·d^(-1))和PFOA+Mor组(PFOA 10mg·kg^(-1)·d^(-1)+Mor 75mg·kg^(-1)·d^(-1)),每组6只。连续灌胃14d。分别检测血清谷丙转氨酶、谷草转氨酶和碱性磷酸酶的活性,肝组织匀浆中丙二醛、C-反应蛋白、白细胞介素-6的水平,超氧化物歧化酶、过氧化氢酶和环氧化酶-2的活性。结果与对照组比较,PFOA组、PFOA+Mor组小鼠血清谷丙转氨酶、谷草转氨酶、碱性磷酸酶活性及PFOA组小鼠肝组织匀浆中丙二醛、C-反应蛋白、白细胞介素-6水平和环氧化酶-2活性均明显升高,PFOA组、PFOA+Mor组小鼠肝组织匀浆中超氧化物歧化酶、过氧化氢酶活性均明显降低(均P<0.05);与PFOA组比较,Mor组、PFOA+Mor组小鼠血清谷丙转氨酶、谷草转氨酶和碱性磷酸酶活性,肝组织匀浆中丙二醛、C-反应蛋白、白细胞介素-6水平和环氧化酶-2活性均明显降低,肝组织匀浆中超氧化物歧化酶、过氧化氢酶活性均明显升高(均P<0.05)。结论桑色素对PFOA诱导的小鼠肝损伤具有保护作用,其机制可能与抑制氧化应激和炎症反应有关。

Objective To investigate the protective effect of morin(Mor) on perfluorooctanoic acid(PFOA)-induced liver injury in mice. Methods Twenty-four male mice were randomly divided into four groups: control group(same volume of normal saline),PFOA group(PFOA 10 mg ?kg'1 ? d_1),M or group(Mor 75 mg ? kg-1 ? d_1) and PFOA + Mor group(PFOA 10 mg ? kg-1 ?d—1+Mor 75 mg ? kg—1 ? d—1 ) , with 6 mice in each group. After treatment for 14 consecutive days, serum activities of alanine transaminase (A L T ), aspartate transaminase (A ST) and alkaline phosphatase( ALP) , hepatic contents of malondialdehyde(MDA) , and hepatic activities of superoxide dismutase (SOD) , catalase (CAT) , C-reactive protein (CRP) , interleukin-6 (IL-6) and cyclooxygenase-2(COX-2) were measured in all groups. Results Compared with control group, liver injury indicators ALT, AST and ALP, inflammatory response markers CRP, IL-6 and COX-2, and lipid peroxidation product MDA increased, but antioxidant enzymes SOD and CAT decreased in PFOA group(P<CO. 05). Compared with PFOA group, simultaneous supplementation of Mor decreased serum activities of ALT, AST and ALP,reduced hepatic contents of MDA,CRP,IL-6 and COX-2, and increased hepatic activities of SOD and CAT(P<C0. 05). Conclusion Mor protects against PFOA-induced liver injury in mice through anti-oxidation and anti-inflammation.