运动对睡眠呼吸暂停模型大鼠血压及肾素血管紧张素系统的影响

Effect of Exercise on Blood Pressure and Renin-Angiotensin System in a Rat Model of Sleep Apnea

目的探讨运动对睡眠呼吸暂停模型SD大鼠肾素血管紧张素系统及血压的影响。方法选取SD雄性3月龄大鼠32只,按随机数字表法分为4组,分别为睡眠呼吸暂停组、运动睡眠呼吸暂停组、运动对照组及空白对照组,每组8只。采用间歇性缺氧、多平台水环境睡眠剥夺法构建睡眠呼吸暂停动物模型,采用中等强度跑台运动进行干预,实验时间8周。实验终点测量大鼠有创动脉压,取血测定血浆肾素、血管紧张素Ⅱ、醛固酮水平。结果各组间有创动脉压比较差异有统计学意义(P<0.05),两两比较显示睡眠呼吸暂停组有创动脉压高于其余3组,差异均有统计学意义(P<0.05),运动睡眠呼吸暂停组有创动脉压低于睡眠呼吸暂停组(P<0.05),高于运动组和空白对照组(P<0.05),运动组和空白对照组2组间差异无统计学意义(P>0.05);各组间血浆肾素水平差异有统计学意义(P<0.05),两两比较显示睡眠呼吸暂停组高于其余3组,差异均有统计学意义(P<0.05),运动睡眠呼吸暂停组血浆肾素水平低于睡眠呼吸暂停组(P<0.05),高于运动对照组(P<0.05),也高于空白对照组(P<0.05),而运动对照组和空白对照组差异无统计学意义(P>0.05);各组间血管紧张素Ⅱ差异有统计学意义(P<0.05),两两比较显示睡眠呼吸暂停组显著高于其余3组,差异均有统计学意义(P<0.01),运动睡眠呼吸暂停组血管紧张素Ⅱ水平低于睡眠呼吸暂停组(P<0.05),高于其余2组,两两比较显示和运动对照组差异有统计学意义(P<0.05),其他组间比较差异均无统计学意义(P>0.05);各组间醛固酮水平比较差异有统计学意义(P<0.05),两两比较显示睡眠呼吸暂停组高于其余3组,差异均有统计学意义(P<0.05),其他组间比较差异均无统计学意义(P>0.05)。结论运动能抑制睡眠呼吸暂停综合征模型大鼠的血压升高,抑制肾素血管紧张素系统。

Objective To investigate the effect of exercise on blood pressure and renin-angiotensin system in a rat model of sleep apnea. Methods Thirty-two young male SD rats were randomly divided into four groups: sleep apnea group, exercise intervention group, exercise control group and blank control group,with 8 rats in each group. The sleep apnea model was established by intermittent hypoxia exposure and multi-platform sleep deprivation. Exercise intervention was carried out with moderate intensity treadmill exercise. The duration of experiment was 8 weeks.Invasive arterial blood pressure and plasma levels of rennin, angiotonin H and aldosterone were measured at the end of the experiment. Results The invasive arterial blood pressure in exercise intervention group was lower than that in sleep apnea group(P<C〇. 05) ,but higher than that in exercise control group or blank control group(P<C〇. 05). There was no significant difference in invasive arterial blood pressure between exercise control group and blank control group ( P >0. 05). The levels of plasma rennin and angiotonin H in exercise intervention group were lower than those in sleep apnea group,but higher than those in exercise control group(P<C〇. 05). There were no significant differences in plasma rennin and angiotonin H levels between exercise intervention group and blank control group,as well as between exercise control group and blank control group(P>0. 05). The levels of aldosterone in sleep apnea group were higher than those in other three groups(_P〈〇. 〇5). There were no significant differences in aldosterone levels among exercise intervention group, exercise control group and blank control group(P>0. 05). Conclusion Exercise can inhibit the increase in blood pressure and reduce plasma levels of renin, angiotensin II and aldosterone in rats with sleep apnea.